Type
PatentPatent Status
Published ApplicationAuthors
Kaur, MandeepEsau, Luke E.
KAUST Department
Computational Bioscience Research Center (CBRC)Technology Transfer
Date
2015-12-24Submitted Date
2015-07-29Permanent link to this record
http://hdl.handle.net/10754/595597
Metadata
Show full item recordAbstract
In one aspect, a method for detaching adherent cells can include adding a cell lifting solution to the media including a sample of adherent cells and incubating the sample of adherent cells with the cell lifting solution. No scraping or pipetting is needed to facilitate cell detachment. The method do not require inactivation of cell lifting solution and no washing of detaching cells is required to remove cell lifting solution. Detached cells can be stained with dye in the presence of cell lifting solution and are further analyzed using flow cytometer. The method has been tested using 6 different cell lines, 4 different assays, two different plate formats (96 and 384 well plates) and two different flow cytometry instruments. The method is simple to perform, less time consuming, with no cell loss and makes high throughput flow cytometry on adherent cells a reality.Application Number
US 20150368620 A1Additional Links
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