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    Method of detaching adherent cells for flow cytometry

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    20150368620.pdf
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    2.187Mb
    Format:
    PDF
    Description:
    Patent
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    Type
    Patent
    Patent Status
    Published Application
    Authors
    Kaur, Mandeep
    Esau, Luke E.
    Assignee
    King Abdullah University of Science and Technology
    KAUST Department
    Computational Bioscience Research Center (CBRC)
    Technology Transfer
    Date
    2015-12-24
    Submitted Date
    2015-07-29
    Permanent link to this record
    http://hdl.handle.net/10754/595597
    
    Metadata
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    Abstract
    In one aspect, a method for detaching adherent cells can include adding a cell lifting solution to the media including a sample of adherent cells and incubating the sample of adherent cells with the cell lifting solution. No scraping or pipetting is needed to facilitate cell detachment. The method do not require inactivation of cell lifting solution and no washing of detaching cells is required to remove cell lifting solution. Detached cells can be stained with dye in the presence of cell lifting solution and are further analyzed using flow cytometer. The method has been tested using 6 different cell lines, 4 different assays, two different plate formats (96 and 384 well plates) and two different flow cytometry instruments. The method is simple to perform, less time consuming, with no cell loss and makes high throughput flow cytometry on adherent cells a reality.
    Application Number
    US 20150368620 A1
    Additional Links
    http://appft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PG01&p=1&u=%2Fnetahtml%2FPTO%2Fsrchnum.html&r=1&f=G&l=50&s1=%2220150368620%22.PGNR.&OS=DN/20150368620&RS=DN/20150368620
    http://assignment.uspto.gov/#/search?adv=publNum:20150368620
    http://www.google.com/patents/US20150333282
    http://worldwide.espacenet.com/publicationDetails/biblio?CC=US&NR=2015333282A1&KC=A1&FT=D
    Collections
    Patents; Computational Bioscience Research Center (CBRC)

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