Nutrient Limitation in Surface Waters of the Oligotrophic Eastern Mediterranean Sea: an Enrichment Microcosm Experiment
Online Publication Date2015-12-01
Print Publication Date2016-04
Permanent link to this recordhttp://hdl.handle.net/10754/594983
MetadataShow full item record
AbstractThe growth rates of planktonic microbes in the pelagic zone of the Eastern Mediterranean Sea are nutrient limited, but the type of limitation is still uncertain. During this study, we investigated the occurrence of N and P limitation among different groups of the prokaryotic and eukaryotic (pico-, nano-, and micro-) plankton using a microcosm experiment during stratified water column conditions in the Cretan Sea (Eastern Mediterranean). Microcosms were enriched with N and P (either solely or simultaneously), and the PO4 turnover time, prokaryotic heterotrophic activity, primary production, and the abundance of the different microbial components were measured. Flow cytometric and molecular fingerprint analyses showed that different heterotrophic prokaryotic groups were limited by different nutrients; total heterotrophic prokaryotic growth was limited by P, but only when both N and P were added, changes in community structure and cell size were detected. Phytoplankton were N and P co-limited, with autotrophic pico-eukaryotes being the exception as they increased even when only P was added after a 2-day time lag. The populations of Synechococcus and Prochlorococcus were highly competitive with each other; Prochlorococcus abundance increased during the first 2 days of P addition but kept increasing only when both N and P were added, whereas Synechococcus exhibited higher pigment content and increased in abundance 3 days after simultaneous N and P additions. Dinoflagellates also showed opportunistic behavior at simultaneous N and P additions, in contrast to diatoms and coccolithophores, which diminished in all incubations. High DNA content viruses, selective grazing, and the exhaustion of N sources probably controlled the populations of diatoms and coccolithophores.
CitationNutrient Limitation in Surface Waters of the Oligotrophic Eastern Mediterranean Sea: an Enrichment Microcosm Experiment 2015 Microbial Ecology
- Impact of nutrient enrichment on productivity of coastal water along the SE Mediterranean shore of Israel - A bioassay approach.
- Authors: Rahav E, Raveh O, Hazan O, Gordon N, Kress N, Silverman J, Herut B
- Issue date: 2018 Feb
- The Temporal Dynamics of Coastal Phytoplankton and Bacterioplankton in the Eastern Mediterranean Sea.
- Authors: Raveh O, David N, Rilov G, Rahav E
- Issue date: 2015
- Distribution of microbial populations and their relationship with environmental parameters in the coastal waters of Qingdao, China.
- Authors: Wang M, Liang Y, Bai X, Jiang X, Wang F, Qiao Q
- Issue date: 2010 Jul
- Functional responses of prokaryotes and viruses to grazer effects and nutrient additions in freshwater microcosms.
- Authors: Pradeep Ram AS, Sime-Ngando T
- Issue date: 2008 May
- Sulfonate-based networks between eukaryotic phytoplankton and heterotrophic bacteria in the surface ocean.
- Authors: Durham BP, Boysen AK, Carlson LT, Groussman RD, Heal KR, Cain KR, Morales RL, Coesel SN, Morris RM, Ingalls AE, Armbrust EV
- Issue date: 2019 Oct