Nano-mole scale sequential signal assignment by 1 H-detected protein solid-state NMR
Type
ArticleAuthors
Wang, SonglinParthasarathy, Sudhakar
Xiao, Yiling
Nishiyama, Yusuke
Long, Fei
Matsuda, Isamu
Endo, Yuki
Nemoto, Takahiro
Yamauchi, Kazuo
Asakura, Tetsuo
Takeda, Mitsuhiro
Terauchi, Tsutomu
Kainosho, Masatsune
Ishii, Yoshitaka
KAUST Department
Imaging and Characterization Core LabDate
2015Permanent link to this record
http://hdl.handle.net/10754/594287
Metadata
Show full item recordAbstract
We present a 3D 1H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D 13C-detected SSNMR approach. This journal is © The Royal Society of Chemistry 2015.Citation
Wang S, Parthasarathy S, Xiao Y, Nishiyama Y, Long F, et al. (2015) Nano-mole scale sequential signal assignment by 1 H-detected protein solid-state NMR . Chem Commun 51: 15055–15058. Available: http://dx.doi.org/10.1039/c5cc04618a.Sponsors
National Institute of General Medical Sciences[9R01GM098033]Division of Chemistry[CHE 1310363, CHE 957793]
National Institutes of Health[1S10 RR025105]
Publisher
Royal Society of Chemistry (RSC)Journal
Chem. Commun.ISSN
1359-73451364-548X
PubMed ID
26317132ae974a485f413a2113503eed53cd6c53
10.1039/c5cc04618a