Nano-mole scale sequential signal assignment by 1 H-detected protein solid-state NMR
Type
ArticleAuthors
Wang, SonglinParthasarathy, Sudhakar
Xiao, Yiling
Nishiyama, Yusuke
Long, Fei
Matsuda, Isamu
Endo, Yuki
Nemoto, Takahiro
Yamauchi, Kazuo
Asakura, Tetsuo
Takeda, Mitsuhiro
Terauchi, Tsutomu
Kainosho, Masatsune
Ishii, Yoshitaka
KAUST Department
Imaging and Characterization Core LabDate
2015
Metadata
Show full item recordAbstract
We present a 3D 1H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D 13C-detected SSNMR approach. This journal is © The Royal Society of Chemistry 2015.Citation
Wang S, Parthasarathy S, Xiao Y, Nishiyama Y, Long F, et al. (2015) Nano-mole scale sequential signal assignment by 1 H-detected protein solid-state NMR . Chem Commun 51: 15055–15058. Available: http://dx.doi.org/10.1039/c5cc04618a.Sponsors
National Institute of General Medical Sciences[9R01GM098033]Division of Chemistry[CHE 1310363, CHE 957793]
National Institutes of Health[1S10 RR025105]
Publisher
Royal Society of Chemistry (RSC)Journal
Chem. Commun.ISSN
1359-73451364-548X
PubMed ID
26317132Handle URI
http://hdl.handle.net/10754/594287ae974a485f413a2113503eed53cd6c53
10.1039/c5cc04618a
Scopus Count
Related articles
- Nano-mole scale side-chain signal assignment by 1H-detected protein solid-state NMR by ultra-fast magic-angle spinning and stereo-array isotope labeling.
- Authors: Wang S, Parthasarathy S, Nishiyama Y, Endo Y, Nemoto T, Yamauchi K, Asakura T, Takeda M, Terauchi T, Kainosho M, Ishii Y
- Issue date: 2015
- Spectral editing at ultra-fast magic-angle-spinning in solid-state NMR: facilitating protein sequential signal assignment by HIGHLIGHT approach.
- Authors: Wang S, Matsuda I, Long F, Ishii Y
- Issue date: 2016 Feb
- Progress in proton-detected solid-state NMR (SSNMR): Super-fast 2D SSNMR collection for nano-mole-scale proteins.
- Authors: Ishii Y, Wickramasinghe A, Matsuda I, Endo Y, Ishii Y, Nishiyama Y, Nemoto T, Kamihara T
- Issue date: 2018 Jan
- Sensitivity and resolution enhanced solid-state NMR for paramagnetic systems and biomolecules under very fast magic angle spinning.
- Authors: Parthasarathy S, Nishiyama Y, Ishii Y
- Issue date: 2013 Sep 17
- Ultra fast magic angle spinning solid - state NMR spectroscopy of intact bone.
- Authors: Singh C, Rai RK, Kayastha AM, Sinha N
- Issue date: 2016 Feb