Show simple item record

dc.contributor.authorHudik, Elodie
dc.contributor.authorYoshioka, Yasushi
dc.contributor.authorDomenichini, SéVerine
dc.contributor.authorBourge, Mickaël
dc.contributor.authorSoubigout-Taconnat, Ludivine
dc.contributor.authorMazubert, Christelle
dc.contributor.authorYi, Dalong
dc.contributor.authorBujaldon, Sandrine
dc.contributor.authorHayashi, Hiroyuki
dc.contributor.authorDe Veylder, Lieven
dc.contributor.authorBergounioux, Catherine
dc.contributor.authorBenhamed, Moussa
dc.contributor.authorRaynaud, Cécile
dc.date.accessioned2016-01-19T14:43:58Z
dc.date.available2016-01-19T14:43:58Z
dc.date.issued2014-07-18
dc.identifier.citationHudik E, Yoshioka Y, Domenichini S, Bourge M, Soubigout-Taconnat L, et al. (2014) Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutant . Plant Physiol 166: 152–167. Available: http://dx.doi.org/10.1104/pp.114.242628.
dc.identifier.issn0032-0889
dc.identifier.issn1532-2548
dc.identifier.pmid25037213
dc.identifier.doi10.1104/pp.114.242628
dc.identifier.urihttp://hdl.handle.net/10754/594233
dc.description.abstractThe majority of research on cell cycle regulation is focused on the nuclear events that govern the replication and segregation of the genome between the two daughter cells. However, eukaryotic cells contain several compartmentalized organelles with specialized functions, and coordination among these organelles is required for proper cell cycle progression, as evidenced by the isolation of several mutants in which both organelle function and overall plant development were affected. To investigate how chloroplast dysfunction affects the cell cycle, we analyzed the crumpled leaf (crl) mutant of Arabidopsis (Arabidopsis thaliana), which is deficient for a chloroplastic protein and displays particularly severe developmental defects. In the crl mutant, we reveal that cell cycle regulation is altered drastically and that meristematic cells prematurely enter differentiation, leading to reduced plant stature and early endoreduplication in the leaves. This response is due to the repression of several key cell cycle regulators as well as constitutive activation of stress-response genes, among them the cell cycle inhibitor SIAMESE-RELATED5. One unique feature of the crl mutant is that it produces aplastidic cells in several organs, including the root tip. By investigating the consequence of the absence of plastids on cell cycle progression, we showed that nuclear DNA replication occurs in aplastidic cells in the root tip, which opens future research prospects regarding the dialogue between plastids and the nucleus during cell cycle regulation in higher plants.
dc.description.sponsorshipThis work was supported by the Agence Nationale de la Recherche of France (grant no. ANR 2010 JCJC1207 01) and the Interuniversity Attraction Poles Program (grant no. IUAP P7/29 MARS), initiated by the Belgian Science Policy Office, as well as by the facilities and expertise of the Imagif Cell Biology Unit of the Gif Campus, which is supported by the Infrastructures en Biologie Sante et Agronomie, the French National Research Agency, under Investments for the Future programs France-BioImaging Infrastructure (grant no. ANR-10-INSB-04-01), Saclay Plant Sciences (grant no. ANR-10-LABX-0040-SPS), and the Conseil General de l'Essonne.
dc.publisherAmerican Society of Plant Biologists (ASPB)
dc.titleChloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutant
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalPlant Physiology
dc.identifier.pmcidPMC4149703
dc.contributor.institutionInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Centre National de la Recherche Scientifique Université-Paris Sud, Laboratoire d’Excellence Saclay Plant ScienceOrsay, France
dc.contributor.institutionDivision of Biological Science, Graduate School of Science, Nagoya UniversityChikusa-ku, Nagoya, Japan
dc.contributor.institutionFédération de Recherche de Gif FRC3115, Pôle de Biologie CellulaireGif-sur-Yvette, France
dc.contributor.institutionUnité de Recherche en Génomique VégétaleEvry, France
dc.contributor.institutionDepartment of Plant Systems Biology, Vlaams Instituut voor BiotechnologieGhent, Belgium
dc.contributor.institutionDepartment of Plant Biotechnology and Bioinformatics, Ghent UniversityGhent, Belgium
dc.contributor.institutionCentre National de la Recherche Scientifique, Unité Mixte de Recherche 7141, Laboratoire de Physiologie Membranaire et Moléculaire du Chloroplaste,Institut de Biologie Physico-ChimiqueParis, France
dc.contributor.institutionDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of TokyoBunkyo-ku, Tokyo, Japan
kaust.personHudik, Elodie
kaust.personBenhamed, Moussa


This item appears in the following Collection(s)

Show simple item record