Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutant
De Veylder, Lieven
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Chromatin and development Research Group
Online Publication Date2014-07-18
Print Publication Date2014-09
Permanent link to this recordhttp://hdl.handle.net/10754/594233
MetadataShow full item record
AbstractThe majority of research on cell cycle regulation is focused on the nuclear events that govern the replication and segregation of the genome between the two daughter cells. However, eukaryotic cells contain several compartmentalized organelles with specialized functions, and coordination among these organelles is required for proper cell cycle progression, as evidenced by the isolation of several mutants in which both organelle function and overall plant development were affected. To investigate how chloroplast dysfunction affects the cell cycle, we analyzed the crumpled leaf (crl) mutant of Arabidopsis (Arabidopsis thaliana), which is deficient for a chloroplastic protein and displays particularly severe developmental defects. In the crl mutant, we reveal that cell cycle regulation is altered drastically and that meristematic cells prematurely enter differentiation, leading to reduced plant stature and early endoreduplication in the leaves. This response is due to the repression of several key cell cycle regulators as well as constitutive activation of stress-response genes, among them the cell cycle inhibitor SIAMESE-RELATED5. One unique feature of the crl mutant is that it produces aplastidic cells in several organs, including the root tip. By investigating the consequence of the absence of plastids on cell cycle progression, we showed that nuclear DNA replication occurs in aplastidic cells in the root tip, which opens future research prospects regarding the dialogue between plastids and the nucleus during cell cycle regulation in higher plants.
CitationHudik E, Yoshioka Y, Domenichini S, Bourge M, Soubigout-Taconnat L, et al. (2014) Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutant . Plant Physiol 166: 152–167. Available: http://dx.doi.org/10.1104/pp.114.242628.
SponsorsThis work was supported by the Agence Nationale de la Recherche of France (grant no. ANR 2010 JCJC1207 01) and the Interuniversity Attraction Poles Program (grant no. IUAP P7/29 MARS), initiated by the Belgian Science Policy Office, as well as by the facilities and expertise of the Imagif Cell Biology Unit of the Gif Campus, which is supported by the Infrastructures en Biologie Sante et Agronomie, the French National Research Agency, under Investments for the Future programs France-BioImaging Infrastructure (grant no. ANR-10-INSB-04-01), Saclay Plant Sciences (grant no. ANR-10-LABX-0040-SPS), and the Conseil General de l'Essonne.
PubMed Central IDPMC4149703
- The Arabidopsis minD mutation causes aberrant FtsZ1 ring placement and moderate heterogeneity of chloroplasts in the leaf epidermis.
- Authors: Fujiwara MT, Yasuzawa M, Sasaki S, Nakano T, Niwa Y, Yoshida S, Abe T, Itoh RD
- Issue date: 2017 Jul 3
- Plant cells without detectable plastids are generated in the crumpled leaf mutant of Arabidopsis thaliana.
- Authors: Chen Y, Asano T, Fujiwara MT, Yoshida S, Machida Y, Yoshioka Y
- Issue date: 2009 May
- A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis.
- Authors: Asano T, Yoshioka Y, Kurei S, Sakamoto W, Machida Y, Sodmergen
- Issue date: 2004 May
- CRUMPLED LEAF (CRL) homologs of Physcomitrella patens are involved in the complete separation of dividing plastids.
- Authors: Sugita C, Kato Y, Yoshioka Y, Tsurumi N, Iida Y, Machida Y, Sugita M
- Issue date: 2012 Jun
- The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis.
- Authors: Hricová A, Quesada V, Micol JL
- Issue date: 2006 Jul