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dc.contributor.authorElshenawy, Mohamed
dc.contributor.authorJergic, Slobodan
dc.contributor.authorXu, Zhi Qiang
dc.contributor.authorSobhy, Mohamed Abdelmaboud
dc.contributor.authorTakahashi, Masateru
dc.contributor.authorOakley, Aaron J.
dc.contributor.authorDixon, Nicholas E.
dc.contributor.authorHamdan, Samir
dc.date.accessioned2016-01-19T13:22:22Z
dc.date.available2016-01-19T13:22:22Z
dc.date.issued2015-08-31
dc.identifier.citationElshenawy MM, Jergic S, Xu Z-Q, Sobhy MA, Takahashi M, et al. (2015) Replisome speed determines the efficiency of the Tus−Ter replication termination barrier. Nature 525: 394–398. Available: http://dx.doi.org/10.1038/nature14866.
dc.identifier.issn0028-0836
dc.identifier.issn1476-4687
dc.identifier.pmid26322585
dc.identifier.doi10.1038/nature14866
dc.identifier.urihttp://hdl.handle.net/10754/594133
dc.description.abstractIn all domains of life, DNA synthesis occurs bidirectionally from replication origins. Despite variable rates of replication fork progression, fork convergence often occurs at specific sites. Escherichia coli sets a 'replication fork trap' that allows the first arriving fork to enter but not to leave the terminus region. The trap is set by oppositely oriented Tus-bound Ter sites that block forks on approach from only one direction. However, the efficiency of fork blockage by Tus-Ter does not exceed 50% in vivo despite its apparent ability to almost permanently arrest replication forks in vitro. Here we use data from single-molecule DNA replication assays and structural studies to show that both polarity and fork-arrest efficiency are determined by a competition between rates of Tus displacement and rearrangement of Tus-Ter interactions that leads to blockage of slower moving replisomes by two distinct mechanisms. To our knowledge this is the first example where intrinsic differences in rates of individual replisomes have different biological outcomes. ©2015 Macmillan Publishers Limited. All rights reserved.
dc.description.sponsorshipDP0877658, ARC, Australian Research Council; DP0984797, ARC, Australian Research Council; KAUST, Australian Research Council; Australian Research Council
dc.publisherSpringer Nature
dc.titleReplisome speed determines the efficiency of the Tus−Ter replication termination barrier
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalNature
dc.contributor.institutionCentre for Medical and Molecular Bioscience, Illawarra Health and Medical Research Institute, University of WollongongNSW, Australia
kaust.personElshenawy, Mohamed
kaust.personSobhy, Mohamed Abdelmaboud
kaust.personTakahashi, Masateru
kaust.personHamdan, Samir
dc.date.published-online2015-08-31
dc.date.published-print2015-09


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