Show simple item record

dc.contributor.authorPearson, Gareth A
dc.contributor.authorLago-Leston, Asuncion
dc.contributor.authorCánovas, Fernando
dc.contributor.authorCox, Cymon J
dc.contributor.authorVerret, Frederic
dc.contributor.authorLasternas, Sebastian
dc.contributor.authorDuarte, Carlos M.
dc.contributor.authorAgusti, Susana
dc.contributor.authorSerrão, Ester A
dc.date.accessioned2016-01-19T13:22:01Z
dc.date.available2016-01-19T13:22:01Z
dc.date.issued2015-04-14
dc.identifier.citationPearson GA, Lago-Leston A, Cánovas F, Cox CJ, Verret F, et al. (2015) Metatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula. ISME J 9: 2275–2289. Available: http://dx.doi.org/10.1038/ismej.2015.40.
dc.identifier.issn1751-7362
dc.identifier.issn1751-7370
dc.identifier.pmid25871931
dc.identifier.doi10.1038/ismej.2015.40
dc.identifier.urihttp://hdl.handle.net/10754/594118
dc.description.abstractFunctional genomics of diatom-dominated communities fromthe Antarctic Peninsula was studied using comparative metatranscriptomics. Samples obtained from diatom-rich communities in the Bransfield Strait, the western Weddell Sea and sea ice in the Bellingshausen Sea/Wilkins Ice Shelf yielded more than 500K pyrosequencing reads that were combined to produce a global metatranscriptome assembly. Multi-gene phylogenies recovered three distinct communities, and diatom-assigned contigs further indicated little read-sharing between communities, validating an assembly-based annotation and analysis approach. Although functional analysis recovered a core of abundant shared annotations that were expressed across the three diatom communities, over 40% of annotations (but accounting for <10% of sequences) were community-specific. The two pelagic communities differed in their expression of N-metabolism and acquisition genes, which was almost absent in post-bloom conditions in the Weddell Sea community, while enrichment of transporters for ammonia and urea in Bransfield Strait diatoms suggests a physiological stance towards acquisition of reduced N-sources. The depletion of carbohydrate and energy metabolism pathways in sea ice relative to pelagic communities, together with increased light energy dissipation (via LHCSR proteins), photorespiration, and NO3 - uptake and utilization all pointed to irradiance stress and/or inorganic carbon limitation within sea ice. Ice-binding proteins and cold-shock transcription factors were also enriched in sea ice diatoms. Surprisingly, the abundance of gene transcripts for the translational machinery tracked decreasing environmental temperature across only a 4 °C range, possibly reflecting constraints on translational efficiency and protein production in cold environments. © 2015 International Society for Microbial Ecology All rights reserved.
dc.publisherSpringer Nature
dc.titleMetatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalThe ISME Journal
dc.identifier.pmcidPMC4579479
dc.contributor.institutionCentre of Marine Sciences, University of the Algarve, Faro, Portugal
dc.contributor.institutionInstitute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Crete, Greece
dc.contributor.institutionGlobal Change Department, IMEDEA (CSIC-UIB), Instituto Mediterráneo de Estudios Avanzados, Esporles, Mallorca, Spain
kaust.personDuarte, Carlos M.
kaust.personAgusti, Susana
dc.date.published-online2015-04-14
dc.date.published-print2015-10


This item appears in the following Collection(s)

Show simple item record