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dc.contributor.authorKovács, Krisztián A.
dc.contributor.authorSteinmann, Myriam
dc.contributor.authorHalfon, Olivier
dc.contributor.authorMagistretti, Pierre J.
dc.contributor.authorCardinaux, Jean-René
dc.date.accessioned2016-01-19T13:21:44Z
dc.date.available2016-01-19T13:21:44Z
dc.date.issued2015-11
dc.identifier.citationKovács KA, Steinmann M, Halfon O, Magistretti PJ, Cardinaux J-R (2015) Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2. Cellular Signalling 27: 2252–2260. Available: http://dx.doi.org/10.1016/j.cellsig.2015.08.001.
dc.identifier.issn0898-6568
dc.identifier.pmid26247811
dc.identifier.doi10.1016/j.cellsig.2015.08.001
dc.identifier.urihttp://hdl.handle.net/10754/594104
dc.description.abstractCREB-binding protein (CBP) and p300 are transcriptional coactivators involved in numerous biological processes that affect cell growth, transformation, differentiation, and development. In this study, we provide evidence of the involvement of homeodomain-interacting protein kinase 2 (HIPK2) in the regulation of CBP activity. We show that HIPK2 interacts with and phosphorylates several regions of CBP. We demonstrate that serines 2361, 2363, 2371, 2376, and 2381 are responsible for the HIPK2-induced mobility shift of CBP C-terminal activation domain. Moreover, we show that HIPK2 strongly potentiates the transcriptional activity of CBP. However, our data suggest that HIPK2 activates CBP mainly by counteracting the repressive action of cell cycle regulatory domain 1 (CRD1), located between amino acids 977 and 1076, independently of CBP phosphorylation. Our findings thus highlight a complex regulation of CBP activity by HIPK2, which might be relevant for the control of specific sets of target genes involved in cellular proliferation, differentiation and apoptosis. © 2015 Elsevier Inc.
dc.description.sponsorshipResearch Executive Agency[291734]
dc.description.sponsorshipSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung[31003A-135692, 3100A0-105773, 3100A0-120699]
dc.publisherElsevier BV
dc.subjectCBP
dc.subjectCoactivator
dc.subjectHIPK2
dc.subjectProtein kinase
dc.subjectTranscriptional regulation
dc.titleComplex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalCellular Signalling
dc.contributor.institutionCenter for Psychiatric Neuroscience, Department of Psychiatry, University Medical Center, University of Lausanne, Prilly, Switzerland
dc.contributor.institutionService of Child and Adolescent Psychiatry, Department of Psychiatry, University Medical Center, University of Lausanne, Lausanne, Switzerland
dc.contributor.institutionInstitute of Science and Technology, Klosterneuburg, Austria
dc.contributor.institutionLaboratory of Neuroenergetics and Cellular Dynamics, Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
dc.contributor.institutionNestlé Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland
kaust.personMagistretti, Pierre J.


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