Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2
Type
ArticleAuthors
Kovács, Krisztián A.Steinmann, Myriam
Halfon, Olivier
Magistretti, Pierre J.

Cardinaux, Jean-René

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Date
2015-11Permanent link to this record
http://hdl.handle.net/10754/594104
Metadata
Show full item recordAbstract
CREB-binding protein (CBP) and p300 are transcriptional coactivators involved in numerous biological processes that affect cell growth, transformation, differentiation, and development. In this study, we provide evidence of the involvement of homeodomain-interacting protein kinase 2 (HIPK2) in the regulation of CBP activity. We show that HIPK2 interacts with and phosphorylates several regions of CBP. We demonstrate that serines 2361, 2363, 2371, 2376, and 2381 are responsible for the HIPK2-induced mobility shift of CBP C-terminal activation domain. Moreover, we show that HIPK2 strongly potentiates the transcriptional activity of CBP. However, our data suggest that HIPK2 activates CBP mainly by counteracting the repressive action of cell cycle regulatory domain 1 (CRD1), located between amino acids 977 and 1076, independently of CBP phosphorylation. Our findings thus highlight a complex regulation of CBP activity by HIPK2, which might be relevant for the control of specific sets of target genes involved in cellular proliferation, differentiation and apoptosis. © 2015 Elsevier Inc.Citation
Kovács KA, Steinmann M, Halfon O, Magistretti PJ, Cardinaux J-R (2015) Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2. Cellular Signalling 27: 2252–2260. Available: http://dx.doi.org/10.1016/j.cellsig.2015.08.001.Sponsors
Research Executive Agency[291734]Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung[31003A-135692, 3100A0-105773, 3100A0-120699]
Publisher
Elsevier BVJournal
Cellular SignallingPubMed ID
26247811ae974a485f413a2113503eed53cd6c53
10.1016/j.cellsig.2015.08.001
Scopus Count
Related articles
- Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2.
- Authors: Hofmann TG, Möller A, Sirma H, Zentgraf H, Taya Y, Dröge W, Will H, Schmitz ML
- Issue date: 2002 Jan
- Regulation of genotoxic stress response by homeodomain-interacting protein kinase 2 through phosphorylation of cyclic AMP response element-binding protein at serine 271.
- Authors: Sakamoto K, Huang BW, Iwasaki K, Hailemariam K, Ninomiya-Tsuji J, Tsuji Y
- Issue date: 2010 Aug 15
- Phosphorylation of ETS transcription factor ER81 in a complex with its coactivators CREB-binding protein and p300.
- Authors: Papoutsopoulou S, Janknecht R
- Issue date: 2000 Oct
- Roles of HIPK1 and HIPK2 in AML1- and p300-dependent transcription, hematopoiesis and blood vessel formation.
- Authors: Aikawa Y, Nguyen LA, Isono K, Takakura N, Tagata Y, Schmitz ML, Koseki H, Kitabayashi I
- Issue date: 2006 Sep 6
- Interaction of PIMT with transcriptional coactivators CBP, p300, and PBP differential role in transcriptional regulation.
- Authors: Misra P, Qi C, Yu S, Shah SH, Cao WQ, Rao MS, Thimmapaya B, Zhu Y, Reddy JK
- Issue date: 2002 May 31