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dc.contributor.authorAnderson, James V.
dc.contributor.authorDoǧramaci, Münevver
dc.contributor.authorHorvath, David P.
dc.contributor.authorFoley, Michael E.
dc.contributor.authorChao, Wun S.
dc.contributor.authorSuttle, Jeffrey C.
dc.contributor.authorThimmapuram, Jyothi
dc.contributor.authorHernandez, Alvaro G.
dc.contributor.authorAli, Shahjahan
dc.contributor.authorMikel, Mark A.
dc.date.accessioned2016-01-19T13:21:08Z
dc.date.available2016-01-19T13:21:08Z
dc.date.issued2012-05-13
dc.identifier.citationAnderson JV, Doğramacı M, Horvath DP, Foley ME, Chao WS, et al. (2012) Auxin and ABA act as central regulators of developmental networks associated with paradormancy in Canada thistle (Cirsium arvense). Funct Integr Genomics 12: 515–531. Available: http://dx.doi.org/10.1007/s10142-012-0280-5.
dc.identifier.issn1438-793X
dc.identifier.issn1438-7948
dc.identifier.pmid22580957
dc.identifier.doi10.1007/s10142-012-0280-5
dc.identifier.urihttp://hdl.handle.net/10754/594081
dc.description.abstractAbstract Dormancy in underground vegetative buds of Canada thistle, an herbaceous perennial weed, allows escape from current control methods and contributes to its invasive nature. In this study, ∼65 % of root sections obtained from greenhouse propagated Canada thistle produced new vegetative shoots by 14 days post-sectioning. RNA samples obtained from sectioned roots incubated 0, 24, 48, and 72 h at 25°C under 16:8 h light-dark conditions were used to construct four MID-tagged cDNA libraries. Analysis of in silico data obtained using Roche 454 GS-FLX pyrosequencing technologies identified molecular networks associated with paradormancy release in underground vegetative buds of Canada thistle. Sequencing of two replicate plates produced ∼2.5 million ESTs with an average read length of 362 bases. These ESTs assembled into 67358 unique sequences (21777 contigs and 45581 singlets) and annotation against the Arabidopsis database identified 15232 unigenes. Among the 15232 unigenes, we identified processes enriched with transcripts involved in plant hormone signaling networks. To follow-up on these results, we examined hormone profiles in roots, which identified changes in abscisic acid (ABA) and ABA metabolites, auxins, and cytokinins post-sectioning. Transcriptome and hormone profiling data suggest that interaction between auxin- and ABA-signaling regulate paradormancy maintenance and release in underground adventitious buds of Canada thistle. Our proposed model shows that sectioning-induced changes in polar auxin transport alters ABA metabolism and signaling, which further impacts gibberellic acid signaling involving interactions between ABA and FUSCA3. Here we report that reduced auxin and ABA-signaling, in conjunction with increased cytokinin biosynthesis post-sectioning supports a model where interactions among hormones drives molecular networks leading to cell division, differentiation, and vegetative outgrowth. ©Springer-Verlag (outside the USA) 2012.
dc.publisherSpringer Nature
dc.subjectCanada thistle
dc.subjectDormancy
dc.subjectESTs
dc.subjectHormone profiling
dc.titleAuxin and ABA act as central regulators of developmental networks associated with paradormancy in Canada thistle (Cirsium arvense)
dc.typeArticle
dc.contributor.departmentBioscience Core Lab
dc.identifier.journalFunctional & Integrative Genomics
dc.contributor.institutionUSDA-Agricultural Research Service, Biosciences Research Laboratory, 1605 Albrecht Blvd., Fargo, ND 58102-2765, United States
dc.contributor.institutionNorthern Crop Science Laboratory, USDA-ARS, 1605 Albrecht Blvd., Fargo, ND 58102-2765, United States
dc.contributor.institutionW.M. Keck Center for Comparative and Functional Genomics, University of Illinois, Urbana, IL, United States
dc.contributor.institutionDepartment of Crop Sciences, Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL, United States
kaust.personAli, Shahjahan
dc.date.published-online2012-05-13
dc.date.published-print2012-08


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