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dc.contributor.authorMahfouz, Magdy M.
dc.contributor.authorPiatek, Agnieszka Anna
dc.contributor.authorStewart, Charles Neal
dc.date.accessioned2016-01-19T13:20:34Z
dc.date.available2016-01-19T13:20:34Z
dc.date.issued2014-09-24
dc.identifier.citationMahfouz MM, Piatek A, Stewart CN (2014) Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives. Plant Biotechnology Journal 12: 1006–1014. Available: http://dx.doi.org/10.1111/pbi.12256.
dc.identifier.issn1467-7644
dc.identifier.pmid25250853
dc.identifier.doi10.1111/pbi.12256
dc.identifier.urihttp://hdl.handle.net/10754/594061
dc.description.abstractThe ability to precisely modify genome sequence and regulate gene expression patterns in a site-specific manner holds much promise in plant biotechnology. Genome-engineering technologies that enable such highly specific and efficient modification are advancing with unprecedented pace. Transcription activator-like effectors (TALEs) provide customizable DNA-binding modules designed to bind to any sequence of interest. Thus, TALEs have been used as a DNA targeting module fused to functional domains for a variety of targeted genomic and epigenomic modifications. TALE nucleases (TALENs) have been used with much success across eukaryotic species to edit genomes. Recently, clustered regularly interspaced palindromic repeats (CRISPRs) that are used as guide RNAs for Cas9 nuclease-specific digestion has been introduced as a highly efficient DNA-targeting platform for genome editing and regulation. Here, we review the discovery, development and limitations of TALENs and CRIPSR/Cas9 systems as genome-engineering platforms in plants. We discuss the current questions, potential improvements and the development of the next-generation genome-editing platforms with an emphasis on producing designer plants to address the needs of agriculture and basic plant biology.
dc.publisherWiley-Blackwell
dc.subjectCRISPR/Cas9 system
dc.subjectDesigner transcriptional regulators
dc.subjectDNA-binding modules
dc.subjectSynthetic site-specific nucleases (SNNs)
dc.subjectTAL effectors
dc.subjectTargeted genome modifications
dc.titleGenome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentBioscience Program
dc.identifier.journalPlant Biotechnology Journal
dc.contributor.institutionDepartment of Plant Sciences; University of Tennessee; Knoxville TN USA
kaust.personMahfouz, Magdy M.
kaust.personPiatek, Agnieszka Anna


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