Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Laboratory for Genome Engineering
Plant Science Program
Online Publication Date2014-09-24
Print Publication Date2014-10
Permanent link to this recordhttp://hdl.handle.net/10754/594061
MetadataShow full item record
AbstractThe ability to precisely modify genome sequence and regulate gene expression patterns in a site-specific manner holds much promise in plant biotechnology. Genome-engineering technologies that enable such highly specific and efficient modification are advancing with unprecedented pace. Transcription activator-like effectors (TALEs) provide customizable DNA-binding modules designed to bind to any sequence of interest. Thus, TALEs have been used as a DNA targeting module fused to functional domains for a variety of targeted genomic and epigenomic modifications. TALE nucleases (TALENs) have been used with much success across eukaryotic species to edit genomes. Recently, clustered regularly interspaced palindromic repeats (CRISPRs) that are used as guide RNAs for Cas9 nuclease-specific digestion has been introduced as a highly efficient DNA-targeting platform for genome editing and regulation. Here, we review the discovery, development and limitations of TALENs and CRIPSR/Cas9 systems as genome-engineering platforms in plants. We discuss the current questions, potential improvements and the development of the next-generation genome-editing platforms with an emphasis on producing designer plants to address the needs of agriculture and basic plant biology.
CitationMahfouz MM, Piatek A, Stewart CN (2014) Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives. Plant Biotechnology Journal 12: 1006–1014. Available: http://dx.doi.org/10.1111/pbi.12256.
JournalPlant Biotechnology Journal
- The CRISPR/Cas9 system for plant genome editing and beyond.
- Authors: Bortesi L, Fischer R
- Issue date: 2015 Jan-Feb
- Plant genome engineering in full bloom.
- Authors: Lozano-Juste J, Cutler SR
- Issue date: 2014 May
- CRISPR/Cas9: an advanced tool for editing plant genomes.
- Authors: Samanta MK, Dey A, Gayen S
- Issue date: 2016 Oct
- [CRISPR/Cas9-based genome editing systems and the analysis of targeted genome mutations in plants].
- Authors: Ma XL, Liu YG
- Issue date: 2016 Feb
- Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research.
- Authors: Liu D, Hu R, Palla KJ, Tuskan GA, Yang X
- Issue date: 2016 Apr