Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Plant Science Program
Desert Agriculture Initiative
MetadataShow full item record
AbstractThe ability to precisely modify genome sequence and regulate gene expression patterns in a site-specific manner holds much promise in plant biotechnology. Genome-engineering technologies that enable such highly specific and efficient modification are advancing with unprecedented pace. Transcription activator-like effectors (TALEs) provide customizable DNA-binding modules designed to bind to any sequence of interest. Thus, TALEs have been used as a DNA targeting module fused to functional domains for a variety of targeted genomic and epigenomic modifications. TALE nucleases (TALENs) have been used with much success across eukaryotic species to edit genomes. Recently, clustered regularly interspaced palindromic repeats (CRISPRs) that are used as guide RNAs for Cas9 nuclease-specific digestion has been introduced as a highly efficient DNA-targeting platform for genome editing and regulation. Here, we review the discovery, development and limitations of TALENs and CRIPSR/Cas9 systems as genome-engineering platforms in plants. We discuss the current questions, potential improvements and the development of the next-generation genome-editing platforms with an emphasis on producing designer plants to address the needs of agriculture and basic plant biology.
CitationMahfouz MM, Piatek A, Stewart CN (2014) Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives. Plant Biotechnology Journal 12: 1006–1014. Available: http://dx.doi.org/10.1111/pbi.12256.
JournalPlant Biotechnology Journal
- Plant genome engineering in full bloom.
- Authors: Lozano-Juste J, Cutler SR
- Issue date: 2014 May
- The CRISPR/Cas9 system for plant genome editing and beyond.
- Authors: Bortesi L, Fischer R
- Issue date: 2015 Jan-Feb
- TALEN or Cas9 - rapid, efficient and specific choices for genome modifications.
- Authors: Wei C, Liu J, Yu Z, Zhang B, Gao G, Jiao R
- Issue date: 2013 Jun 20
- Targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system.
- Authors: Liang Z, Zhang K, Chen K, Gao C
- Issue date: 2014 Feb 20
- Genome editing with engineered nucleases in plants.
- Authors: Osakabe Y, Osakabe K
- Issue date: 2015 Mar