Extracellular DNA amplicon sequencing reveals high levels of benthic eukaryotic diversity in the central Red Sea
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionMarine Science Program
Red Sea Research Center (RSRC)
Date
2015-11-10Online Publication Date
2015-11-10Print Publication Date
2016-04Permanent link to this record
http://hdl.handle.net/10754/594059
Metadata
Show full item recordAbstract
The present study aims to characterize the benthic eukaryotic biodiversity patterns at a coarse taxonomic level in three areas of the central Red Sea (a lagoon, an offshore area in Thuwal and a shallow coastal area near Jeddah) based on extracellular DNA. High-throughput amplicon sequencing targeting the V9 region of the 18S rRNA gene was undertaken for 32 sediment samples. High levels of alpha-diversity were detected with 16,089 operational taxonomic units (OTUs) being identified. The majority of the OTUs were assigned to Metazoa (29.2%), Alveolata (22.4%) and Stramenopiles (17.8%). Stramenopiles (Diatomea) and Alveolata (Ciliophora) were frequent in a lagoon and in shallower coastal stations, whereas metazoans (Arthropoda: Maxillopoda) were dominant in deeper offshore stations. Only 24.6% of total OTUs were shared among all areas. Beta-diversity was generally lower between the lagoon and Jeddah (nearshore) than between either of those and the offshore area, suggesting a nearshore–offshore biodiversity gradient. The current approach allowed for a broad-range of benthic eukaryotic biodiversity to be analysed with significantly less labour than would be required by other traditional taxonomic approaches. Our findings suggest that next generation sequencing techniques have the potential to provide a fast and standardised screening of benthic biodiversity at large spatial and temporal scales.Citation
Pearman JK, Irigoien X, Carvalho S (2015) Extracellular DNA amplicon sequencing reveals high levels of benthic eukaryotic diversity in the central Red Sea. Marine Genomics. Available: http://dx.doi.org/10.1016/j.margen.2015.10.008.Sponsors
King Abdullah University of Science and TechnologyPublisher
Elsevier BVJournal
Marine GenomicsPubMed ID
26525270ae974a485f413a2113503eed53cd6c53
10.1016/j.margen.2015.10.008
Scopus Count
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