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    Extracellular DNA amplicon sequencing reveals high levels of benthic eukaryotic diversity in the central Red Sea

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    Type
    Article
    Authors
    Pearman, John K. cc
    Irigoien, Xabier cc
    Carvalho, Susana cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Marine Science Program
    Red Sea Research Center (RSRC)
    Date
    2015-11-10
    Online Publication Date
    2015-11-10
    Print Publication Date
    2016-04
    Permanent link to this record
    http://hdl.handle.net/10754/594059
    
    Metadata
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    Abstract
    The present study aims to characterize the benthic eukaryotic biodiversity patterns at a coarse taxonomic level in three areas of the central Red Sea (a lagoon, an offshore area in Thuwal and a shallow coastal area near Jeddah) based on extracellular DNA. High-throughput amplicon sequencing targeting the V9 region of the 18S rRNA gene was undertaken for 32 sediment samples. High levels of alpha-diversity were detected with 16,089 operational taxonomic units (OTUs) being identified. The majority of the OTUs were assigned to Metazoa (29.2%), Alveolata (22.4%) and Stramenopiles (17.8%). Stramenopiles (Diatomea) and Alveolata (Ciliophora) were frequent in a lagoon and in shallower coastal stations, whereas metazoans (Arthropoda: Maxillopoda) were dominant in deeper offshore stations. Only 24.6% of total OTUs were shared among all areas. Beta-diversity was generally lower between the lagoon and Jeddah (nearshore) than between either of those and the offshore area, suggesting a nearshore–offshore biodiversity gradient. The current approach allowed for a broad-range of benthic eukaryotic biodiversity to be analysed with significantly less labour than would be required by other traditional taxonomic approaches. Our findings suggest that next generation sequencing techniques have the potential to provide a fast and standardised screening of benthic biodiversity at large spatial and temporal scales.
    Citation
    Pearman JK, Irigoien X, Carvalho S (2015) Extracellular DNA amplicon sequencing reveals high levels of benthic eukaryotic diversity in the central Red Sea. Marine Genomics. Available: http://dx.doi.org/10.1016/j.margen.2015.10.008.
    Sponsors
    King Abdullah University of Science and Technology
    Publisher
    Elsevier BV
    Journal
    Marine Genomics
    DOI
    10.1016/j.margen.2015.10.008
    PubMed ID
    26525270
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.margen.2015.10.008
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC); Marine Science Program; Reef Genomics, part of the Global Ocean Genome Project

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