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dc.contributor.authorKamphuis, L. G.
dc.contributor.authorLichtenzveig, J.
dc.contributor.authorPeng, K.
dc.contributor.authorGuo, S.-M.
dc.contributor.authorKlingler, John
dc.contributor.authorSiddique, K. H. M.
dc.contributor.authorGao, L.-L.
dc.contributor.authorSingh, K. B.
dc.date.accessioned2016-01-13T14:36:35Z
dc.date.available2016-01-13T14:36:35Z
dc.date.issued2013-09-21
dc.identifier.citationCharacterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula 2013, 64 (16):5157 Journal of Experimental Botany
dc.identifier.issn0022-0957
dc.identifier.issn1460-2431
dc.identifier.doi10.1093/jxb/ert305
dc.identifier.urihttp://hdl.handle.net/10754/593396
dc.description.abstractAphids cause significant yield losses in agricultural crops worldwide. Medicago truncatula, a model legume, cultivated pasture species in Australia and close relative of alfalfa (Medicago sativa), was used to study the defence response against Therioaphis trifolii f. maculate [spotted alfalfa aphid (SAA)]. Aphid performance and plant damage were compared among three accessions. A20 is highly susceptible, A17 has moderate resistance, and Jester is strongly resistant. Subsequent analyses using A17 and A20, reciprocal F1s and an A17×A20 recombinant inbred line (RIL) population revealed that this moderate resistance is phloem mediated and involves antibiosis and tolerance but not antixenosis. Electrical penetration graph analysis also identified a novel waveform termed extended potential drop, which occurred following SAA infestation of M. truncatula. Genetic dissection using the RIL population revealed three quantitative trait loci on chromosomes 3, 6, and 7 involved in distinct modes of aphid defence including antibiosis and tolerance. An antibiosis locus resides on linkage group 3 (LG3) and is derived from A17, whereas a plant tolerance and antibiosis locus resides on LG6 and is derived from A20, which exhibits strong temporary tolerance. The loci identified reside in regions harbouring classical resistance genes, and introgression of these loci in current medic cultivars may help provide durable resistance to SAA, while elucidation of their molecular mechanisms may provide valuable insight into other aphid–plant interactions.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.relation.urlhttp://jxb.oxfordjournals.org/lookup/doi/10.1093/jxb/ert305
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.subjectAntibiosis
dc.subjectantixenosis
dc.subjectEPG
dc.subjectherbivory
dc.subjectphloem
dc.subjectquantitative trait locus (QTL)
dc.subjectsap-sucking insect
dc.subjectvein chlorosis
dc.titleCharacterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula
dc.typeArticle
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalJournal of Experimental Botany
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionCSIRO Plant Industry, Private Bag 5, Wembley, WA 6913, Australia
dc.contributor.institutionThe UWA Institute of Agriculture, University of Western Australia, Crawley, WA 6009, Australia
dc.contributor.institutionDepartment of Environment and Agriculture, Curtin University, Bentley, WA 6102, Australia
dc.contributor.institutionKey Laboratory of Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, PR China
dc.contributor.institutionResearch School of Biology, Australian National University, Acton, ACT 0200, Australia
dc.contributor.institutionBoyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personKlingler, J. P.
refterms.dateFOA2018-06-13T19:11:54Z
dc.date.published-online2013-09-21
dc.date.published-print2013-11


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