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Cytotoxicity and intracellular dissolution of nickel nanowires.pdf
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ArticleAuthors
Perez, Jose E.
Contreras, Maria F.

Vidal, Enrique Vilanova
Felix Servin, Laura P.
Margineanu, Michael B.

Luongo, Giovanni
Porter, Alexandra E.
Dunlop, Iain E.
Ravasi, Timothy

Kosel, Jürgen

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Chemical and Biological Engineering Program
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Electrical Engineering Program
Entrepreneurship Center
Date
2016-02-05Online Publication Date
2016-02-05Print Publication Date
2016-08-08Permanent link to this record
http://hdl.handle.net/10754/592811
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The assessment of cytotoxicity of nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates for such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis and electron microscopy. After exposure to the NWs, cytotoxicity was evaluated in terms of cell viability, cell membrane damage and induced apoptosis/necrosis on the model human cell line HCT 116. The influence of NW to cell ratio (10:1 to 1000:1) and exposure times up to 72 hours was analyzed for Ni NWs of 5.4 µm in length, as well as for Ni ions. The results show that cytotoxicity markedly increases past 24 hours of incubation. Cellular uptake of NWs takes place through the phagocytosis pathway, with a fraction of the dose of NWs dissolved inside the cells. Cell death results from a combination of apoptosis and necrosis, where the latter is the outcome of the secondary necrosis pathway. The cytotoxicity of Ni ions and Ni NWs dissolution studies suggest a synergistic toxicity between NW aspect ratio and dissolved Ni, with the cytotoxic effects markedly increasing after 24 hours of incubation.Citation
Cytotoxicity and intracellular dissolution of nickel nanowires 2015:1 NanotoxicologyPublisher
Informa UK LimitedJournal
NanotoxicologyPubMed ID
26692167Additional Links
http://www.tandfonline.com/doi/full/10.3109/17435390.2015.1132343Relations
Is Supplemented By:- [Dataset]
Perez, J. E., Contreras, M. F., Vilanova, E., Felix, L. P., Margineanu, M. B., Luongo, G., … Kosel, J. (2016). Cytotoxicity and intracellular dissolution of nickel nanowires. Figshare. https://doi.org/10.6084/m9.figshare.c.2169857. DOI: 10.6084/M9.FIGSHARE.C.2169857 HANDLE: 10754/624123
Is Supplemented By:
- [Dataset]
. DOI: 10.6084/m9.figshare.1626721 HANDLE: 10754/663599
ae974a485f413a2113503eed53cd6c53
10.3109/17435390.2015.1132343
Scopus Count
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