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dc.contributor.authorDíaz-Sánchez, Violeta
dc.contributor.authorCarmen Limón, M.
dc.contributor.authorSchaub, Patrick
dc.contributor.authorAl-Babili, Salim
dc.contributor.authorAvalos, Javier
dc.date.accessioned2015-12-17T11:54:18Z
dc.date.available2015-12-17T11:54:18Z
dc.date.issued2015-12-16
dc.identifier.citationA RALDH-like enzyme involved in Fusarium verticillioides development 2015 Fungal Genetics and Biology
dc.identifier.issn10871845
dc.identifier.pmid26688466
dc.identifier.doi10.1016/j.fgb.2015.12.005
dc.identifier.urihttp://hdl.handle.net/10754/584052
dc.description.abstractRetinaldehyde dehydrogenases (RALDHs) convert retinal to retinoic acid, an important chordate morphogen. Retinal also occurs in some fungi, such as Fusarium and Ustilago spp., evidenced by the presence of rhodopsins and β–carotene cleaving, retinal-forming dioxygenases. Based on the assumption that retinoic acid may also be formed in fungi, we searched the Fusarium protein databases for RALDHs homologs, focusing on Fusarium verticillioides. Using crude lysates of Escherichia coli cells expressing the corresponding cDNAs, we checked the capability of best matches to convert retinal into retinoic acid in vitro. Thereby, we identified an aldehyde dehydrogenase, termed CarY, as a retinoic acid-forming enzyme, an activity that was also exerted by purified CarY. Targeted mutation of the carY gene in F. verticillioides resulted in alterations of mycelia development and conidia morphology in agar cultures, and reduced capacity to produce perithecia as a female in sexual crosses. Complementation of the mutant with a wild-type carY allele demonstrated that these alterations are caused by the lack of CarY. However, retinoic acid could not be detected by LC-MS analysis either in the wild type or the complemented carY strain in vivo, making elusive the connection between CarY enzymatic activity and retinoic acid formation in the fungus.
dc.language.isoen
dc.publisherElsevier BV
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S1087184515300530
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Fungal Genetics and Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Genetics and Biology, 11 December 2015. DOI: 10.1016/j.fgb.2015.12.005
dc.subjectFusarium verticillioides
dc.subjectRALDH enzyme
dc.subjectRetinoic acid
dc.subjectApocarotenoids
dc.subjectConidia
dc.subjectHyphal development
dc.subjectSexual fertility
dc.titleA RALDH-like enzyme involved in Fusarium verticillioides development
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science Program
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalFungal Genetics and Biology
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Genetics, Faculty of Biology, University of Seville, E–41012 Seville, Spain
dc.contributor.institutionFaculty of Biology, Albert-Ludwigs University of Freiburg, Schaenzlestr. 1, D–79104 Freiburg, Germany
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personAl-Babili, Salim
refterms.dateFOA2016-12-11T00:00:00Z
dc.date.published-online2015-12-16
dc.date.published-print2016-01


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