A RALDH-like enzyme involved in Fusarium verticillioides development
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Plant Science Program
Desert Agriculture Initiative
Online Publication Date2015-12-16
Print Publication Date2016-01
Permanent link to this recordhttp://hdl.handle.net/10754/584052
MetadataShow full item record
AbstractRetinaldehyde dehydrogenases (RALDHs) convert retinal to retinoic acid, an important chordate morphogen. Retinal also occurs in some fungi, such as Fusarium and Ustilago spp., evidenced by the presence of rhodopsins and β–carotene cleaving, retinal-forming dioxygenases. Based on the assumption that retinoic acid may also be formed in fungi, we searched the Fusarium protein databases for RALDHs homologs, focusing on Fusarium verticillioides. Using crude lysates of Escherichia coli cells expressing the corresponding cDNAs, we checked the capability of best matches to convert retinal into retinoic acid in vitro. Thereby, we identified an aldehyde dehydrogenase, termed CarY, as a retinoic acid-forming enzyme, an activity that was also exerted by purified CarY. Targeted mutation of the carY gene in F. verticillioides resulted in alterations of mycelia development and conidia morphology in agar cultures, and reduced capacity to produce perithecia as a female in sexual crosses. Complementation of the mutant with a wild-type carY allele demonstrated that these alterations are caused by the lack of CarY. However, retinoic acid could not be detected by LC-MS analysis either in the wild type or the complemented carY strain in vivo, making elusive the connection between CarY enzymatic activity and retinoic acid formation in the fungus.
CitationA RALDH-like enzyme involved in Fusarium verticillioides development 2015 Fungal Genetics and Biology
JournalFungal Genetics and Biology
- The gene carD encodes the aldehyde dehydrogenase responsible for neurosporaxanthin biosynthesis in Fusarium fujikuroi.
- Authors: Díaz-Sánchez V, Estrada AF, Trautmann D, Al-Babili S, Avalos J
- Issue date: 2011 Sep
- Enzymatic conversion of retinaldehyde to retinoic acid by cloned murine cytosolic and mitochondrial aldehyde dehydrogenases.
- Authors: Chen M, Achkar C, Gudas LJ
- Issue date: 1994 Jul
- FSR1 is essential for virulence and female fertility in Fusarium verticillioides and F. graminearum.
- Authors: Shim WB, Sagaram US, Choi YE, So J, Wilkinson HH, Lee YW
- Issue date: 2006 Jul
- Enzymatic formation of 9-cis, 13-cis, and all-trans retinals from isomers of beta-carotene.
- Authors: Nagao A, Olson JA
- Issue date: 1994 Sep
- Recombinant class I aldehyde dehydrogenases specific for all-trans- or 9-cis-retinal.
- Authors: Montplaisir V, Lan NC, Guimond J, Savineau C, Bhat PV, Mader S
- Issue date: 2002 May 17