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    A RALDH-like enzyme involved in Fusarium verticillioides development

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    Type
    Article
    Authors
    Díaz-Sánchez, Violeta
    Carmen Limón, M.
    Schaub, Patrick
    Al-Babili, Salim cc
    Avalos, Javier cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Center for Desert Agriculture
    Plant Science
    Plant Science Program
    Date
    2015-12-16
    Online Publication Date
    2015-12-16
    Print Publication Date
    2016-01
    Permanent link to this record
    http://hdl.handle.net/10754/584052
    
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    Abstract
    Retinaldehyde dehydrogenases (RALDHs) convert retinal to retinoic acid, an important chordate morphogen. Retinal also occurs in some fungi, such as Fusarium and Ustilago spp., evidenced by the presence of rhodopsins and β–carotene cleaving, retinal-forming dioxygenases. Based on the assumption that retinoic acid may also be formed in fungi, we searched the Fusarium protein databases for RALDHs homologs, focusing on Fusarium verticillioides. Using crude lysates of Escherichia coli cells expressing the corresponding cDNAs, we checked the capability of best matches to convert retinal into retinoic acid in vitro. Thereby, we identified an aldehyde dehydrogenase, termed CarY, as a retinoic acid-forming enzyme, an activity that was also exerted by purified CarY. Targeted mutation of the carY gene in F. verticillioides resulted in alterations of mycelia development and conidia morphology in agar cultures, and reduced capacity to produce perithecia as a female in sexual crosses. Complementation of the mutant with a wild-type carY allele demonstrated that these alterations are caused by the lack of CarY. However, retinoic acid could not be detected by LC-MS analysis either in the wild type or the complemented carY strain in vivo, making elusive the connection between CarY enzymatic activity and retinoic acid formation in the fungus.
    Citation
    A RALDH-like enzyme involved in Fusarium verticillioides development 2015 Fungal Genetics and Biology
    Publisher
    Elsevier BV
    Journal
    Fungal Genetics and Biology
    DOI
    10.1016/j.fgb.2015.12.005
    PubMed ID
    26688466
    Additional Links
    http://linkinghub.elsevier.com/retrieve/pii/S1087184515300530
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.fgb.2015.12.005
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Plant Science Program; Center for Desert Agriculture

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