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dc.contributor.authorZhao, Huayan
dc.contributor.authorLü, Shiyou
dc.contributor.authorLi, Ruixi
dc.contributor.authorChen, Tao
dc.contributor.authorZhang, Huoming
dc.contributor.authorCui, Peng
dc.contributor.authorDing, Feng
dc.contributor.authorLiu, Pei-Nian
dc.contributor.authorWang, Guangchao
dc.contributor.authorXia, Yiji
dc.contributor.authorRunning, Mark P.
dc.contributor.authorXiong, Liming
dc.date.accessioned2015-12-06T12:50:35Z
dc.date.available2015-12-06T12:50:35Z
dc.date.issued2015-08-13
dc.identifier.citationThe Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes 2015, 66 (21):6863 Journal of Experimental Botany
dc.identifier.issn0022-0957
dc.identifier.issn1460-2431
dc.identifier.pmid26272902
dc.identifier.doi10.1093/jxb/erv391
dc.identifier.urihttp://hdl.handle.net/10754/583295
dc.description.abstractThe circularly permuted GTPase large subunit GTPase 1 (LSG1) is involved in the maturation step of the 60S ribosome and is essential for cell viability in yeast. Here, an Arabidopsis mutant dig6 (drought inhibited growth of lateral roots) was isolated. The mutant exhibited multiple auxin-related phenotypes, which included reduced lateral root number, altered leaf veins, and shorter roots. Genetic mapping combined with next-generation DNA sequencing identified that the mutation occurred in AtLSG1-2. This gene was highly expressed in regions of auxin accumulation. Ribosome profiling revealed that a loss of function of AtLSG1-2 led to decreased levels of monosomes, further demonstrating its role in ribosome biogenesis. Quantitative proteomics showed that the expression of certain proteins involved in ribosome biogenesis was differentially regulated, indicating that ribosome biogenesis processes were impaired in the mutant. Further investigations showed that an AtLSG1-2 deficiency caused the alteration of auxin distribution, response, and transport in plants. It is concluded that AtLSG1-2 is integral to ribosome biogenesis, consequently affecting auxin homeostasis and plant development.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.relation.urlhttp://jxb.oxfordjournals.org/lookup/doi/10.1093/jxb/erv391
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.subjectAtLSG1
dc.subjectArabidopsis
dc.subjectauxin homeostasis
dc.subjectexpression pattern
dc.subjectmap-based cloning
dc.subjectproteomics
dc.subjectribosome biogenesis
dc.titleThe Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes
dc.typeArticle
dc.contributor.departmentAnalytical Chemistry Core Lab
dc.contributor.departmentAnalytical Core Lab
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Core Lab
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentImaging and Characterization Core Lab
dc.contributor.departmentPlant Science
dc.identifier.journalJournal of Experimental Botany
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionKey Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
dc.contributor.institutionInstitute for Integrative Genome Biology, Center for Plant Cell Biology & Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
dc.contributor.institutionDepartment of Biology, Hong Kong Baptist University, Hong Kong, P. R. China
dc.contributor.institutionDepartment of Biology, University of Louisville, Louisville, KY 40292, USA
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personZhao, Huayan
kaust.personChen, Tao
kaust.personZhang, Huoming
kaust.personCui, Peng
kaust.personDing, Feng
kaust.personWang, Guangchao
kaust.personXiong, Liming
refterms.dateFOA2018-06-13T16:39:36Z
dc.date.published-online2015-08-13
dc.date.published-print2015-11


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