The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes
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Type
ArticleAuthors
Zhao, HuayanLü, Shiyou
Li, Ruixi
Chen, Tao
Zhang, Huoming

Cui, Peng

Ding, Feng

Liu, Pei-Nian

Wang, Guangchao
Xia, Yiji
Running, Mark P.
Xiong, Liming

KAUST Department
Analytical Chemistry Core LabAnalytical Core Lab
Biological and Environmental Sciences and Engineering (BESE) Division
Bioscience Core Lab
Desert Agriculture Initiative
Imaging and Characterization Core Lab
Plant Science
Date
2015-08-13Online Publication Date
2015-08-13Print Publication Date
2015-11Permanent link to this record
http://hdl.handle.net/10754/583295
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Show full item recordAbstract
The circularly permuted GTPase large subunit GTPase 1 (LSG1) is involved in the maturation step of the 60S ribosome and is essential for cell viability in yeast. Here, an Arabidopsis mutant dig6 (drought inhibited growth of lateral roots) was isolated. The mutant exhibited multiple auxin-related phenotypes, which included reduced lateral root number, altered leaf veins, and shorter roots. Genetic mapping combined with next-generation DNA sequencing identified that the mutation occurred in AtLSG1-2. This gene was highly expressed in regions of auxin accumulation. Ribosome profiling revealed that a loss of function of AtLSG1-2 led to decreased levels of monosomes, further demonstrating its role in ribosome biogenesis. Quantitative proteomics showed that the expression of certain proteins involved in ribosome biogenesis was differentially regulated, indicating that ribosome biogenesis processes were impaired in the mutant. Further investigations showed that an AtLSG1-2 deficiency caused the alteration of auxin distribution, response, and transport in plants. It is concluded that AtLSG1-2 is integral to ribosome biogenesis, consequently affecting auxin homeostasis and plant development.Citation
The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes 2015, 66 (21):6863 Journal of Experimental BotanyPublisher
Oxford University Press (OUP)Journal
Journal of Experimental BotanyPubMed ID
26272902Additional Links
http://jxb.oxfordjournals.org/lookup/doi/10.1093/jxb/erv391ae974a485f413a2113503eed53cd6c53
10.1093/jxb/erv391
Scopus Count
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