Multidrug-Resistant Mycobacterium tuberculosis of the Latin American Mediterranean Lineage, Wrongly Identified as Mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), Causing Active Tuberculosis in South Brazil
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ArticleAuthors
Dalla Costa, Elis R.Vasconcelos, Sidra E. G.
Esteves, Leonardo S.
Gomes, Harrison M.
Gomes, Lia L.

Almeida da Silva, Pedro
Perdigão, João

Portugal, Isabel
Viveiros, Miguel

McNerney, Ruth

Pain, Arnab

Clark, Taane G.

Rastogi, Nalin
Unis, Gisela
Rossetti, Maria Lucia R.
Suffys, Philip Noel
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Date
2015-09-23Online Publication Date
2015-09-23Print Publication Date
2015-12Permanent link to this record
http://hdl.handle.net/10754/583107
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We recently detected the spoligotype patterns of strains of Mycobacterium pinnipedii, a species of the Mycobacterium tuberculosis complex, in sputum samples from nine cases with pulmonary tuberculosis residing in Porto Alegre, South Brazil. Because this species is rarely encountered in humans, we further characterized these nine isolates by additional genotyping techniques, including 24-locus mycobacterial interspersed repetitive-unit–variable-number tandem-repeat (MIRU-VNTR) typing, verification of the loci TbD1, RD9, pks15/1, RDRio, and fbpC, the insertion of IS6110 at a site specific to the M. tuberculosis Latin American Mediterranean (LAM) lineage, and whole-genome sequencing. The combined analysis of these markers revealed that the isolates are in fact M. tuberculosis and more specifically belong to the LAM genotype. Most of these isolates (n = 8) were shown to be multidrug resistant (MDR), which prompted us to perform partial sequencing of the rpoA, rpoB, rpoC, katG, and inhA genes. Seven isolates (77.8%) carried the S315T mutation in katG, and one of these (11%) also presented the C(−17)T single-nucleotide polymorphism (SNP) in inhA. Interestingly, six of the MDR isolates also presented an undescribed insertion of 12 nucleotides (CCA GAA CAA CCC) in codon 516 of rpoB. No putative compensatory mutation was found in either rpoA or rpoC. This is the first report of an M. tuberculosis LAM family strain with a convergent M. pinnipedii spoligotype. These spoligotypes are observed in genotype databases at a modest frequency, highlighting that care must be taken when identifying isolates in the M. tuberculosis complex on the basis of single genetic markers.Citation
Multidrug-Resistant Mycobacterium tuberculosis of the Latin American Mediterranean Lineage, Wrongly Identified as Mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), Causing Active Tuberculosis in South Brazil 2015:JCM.02012-15 Journal of Clinical MicrobiologyPublisher
American Society for MicrobiologyJournal
Journal of Clinical MicrobiologyAdditional Links
http://jcm.asm.org/lookup/doi/10.1128/JCM.02012-15ae974a485f413a2113503eed53cd6c53
10.1128/JCM.02012-15