Show simple item record

dc.contributor.authorAli, Zahir
dc.contributor.authorAbulfaraj, Aala A.
dc.contributor.authorIdris, Ali
dc.contributor.authorAli, Shawkat
dc.contributor.authorTashkandi, Manal
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2015-11-23T13:55:28Z
dc.date.available2015-11-23T13:55:28Z
dc.date.issued2015-11-11
dc.identifier.citationCRISPR/Cas9-mediated viral interference in plants 2015, 16 (1) Genome Biology
dc.identifier.issn1474-760X
dc.identifier.pmid26556628
dc.identifier.doi10.1186/s13059-015-0799-6
dc.identifier.urihttp://hdl.handle.net/10754/582543
dc.description.abstractBackground The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. Results In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. Conclusions These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.urlhttp://genomebiology.com/2015/16/1/238
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.subjectPlant genome engineering
dc.subjectCRISPR/Cas9 system
dc.subjectSynthetic site-specific nucleases
dc.subjectViral-mediated genome editing
dc.subjectVirus resistance
dc.subjectTomato yellow leaf curl virus
dc.subjectTobacco rattle virus
dc.titleCRISPR/Cas9-mediated viral interference in plants
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Science Program
dc.identifier.journalGenome Biology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personAli, Zahir
kaust.personAbulfaraj, Aala Abdulaziz Hussien
kaust.personIdris, Ali
kaust.personAli, Shawkat
kaust.personTashkandi, Manal
kaust.personMahfouz, Magdy M.
dc.relation.issupplementedbyDOI:10.6084/m9.figshare.c.3612878
refterms.dateFOA2018-06-14T03:13:30Z
display.relations<b> Is Supplemented By:</b> <br/> <ul><li><i>[Dataset]</i> <br/> Ali, Z., Aala Abulfaraj, Idris, A., Ali, S., Tashkandi, M., & Magdy Mahfouz. (2015). CRISPR/Cas9-mediated viral interference in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3612878. DOI: <a href="https://doi.org/10.6084/m9.figshare.c.3612878">10.6084/m9.figshare.c.3612878</a> HANDLE: <a href="http://hdl.handle.net/10754/624131">10754/624131</a></li></ul>
dc.date.published-online2015-11-11
dc.date.published-print2015-12


Files in this item

Thumbnail
Name:
s13059-015-0799-6.pdf
Size:
2.006Mb
Format:
PDF
Description:
Main article
Thumbnail
Name:
s13059-015-0799-6-s1.pdf
Size:
2.955Mb
Format:
PDF
Description:
Supplemental files
Thumbnail
Name:
s13059-015-0799-6-s2.pdf
Size:
3.003Mb
Format:
PDF
Description:
Supplemental files
Thumbnail
Name:
s13059-015-0799-6-s3.pdf
Size:
71.02Kb
Format:
PDF
Description:
Supplemental files
Thumbnail
Name:
s13059-015-0799-6-s4.pdf
Size:
2.960Mb
Format:
PDF
Description:
Supplemental files

This item appears in the following Collection(s)

Show simple item record