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    CRISPR/Cas9-mediated viral interference in plants

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    Type
    Article
    Authors
    Ali, Zahir cc
    Abulfaraj, Aala A. cc
    Idris, Ali
    Ali, Shawkat cc
    Tashkandi, Manal cc
    Mahfouz, Magdy M. cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Program
    Center for Desert Agriculture
    Laboratory for Genome Engineering
    Plant Science
    Plant Science Program
    Date
    2015-11-11
    Online Publication Date
    2015-11-11
    Print Publication Date
    2015-12
    Permanent link to this record
    http://hdl.handle.net/10754/582543
    
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    Abstract
    Background The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. Results In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. Conclusions These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.
    Citation
    CRISPR/Cas9-mediated viral interference in plants 2015, 16 (1) Genome Biology
    Publisher
    Springer Nature
    Journal
    Genome Biology
    DOI
    10.1186/s13059-015-0799-6
    PubMed ID
    26556628
    Additional Links
    http://genomebiology.com/2015/16/1/238
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    • [Dataset]
      Ali, Z., Aala Abulfaraj, Idris, A., Ali, S., Tashkandi, M., & Magdy Mahfouz. (2015). CRISPR/Cas9-mediated viral interference in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3612878. DOI: 10.6084/m9.figshare.c.3612878 HANDLE: 10754/624131
    ae974a485f413a2113503eed53cd6c53
    10.1186/s13059-015-0799-6
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Center for Desert Agriculture

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