KAUST DepartmentDesert Agriculture Initiative
Biological and Environmental Sciences and Engineering (BESE) Division
Plant Science Program
Laboratory for Genome Engineering
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AbstractBackground The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. Results In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. Conclusions These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.
CitationCRISPR/Cas9-mediated viral interference in plants 2015, 16 (1) Genome Biology
Is Supplemented ByAli, Z., Aala Abulfaraj, Idris, A., Ali, S., Tashkandi, M., & Magdy Mahfouz. (2015). CRISPR/Cas9-mediated viral interference in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3612878
- Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato.
- Authors: Tashkandi M, Ali Z, Aljedaani F, Shami A, Mahfouz MM
- Issue date: 2018 Oct 5
- RNA interference-based resistance in transgenic tomato plants against Tomato yellow leaf curl virus-Oman (TYLCV-OM) and its associated betasatellite.
- Authors: Ammara Ue, Mansoor S, Saeed M, Amin I, Briddon RW, Al-Sadi AM
- Issue date: 2015 Mar 4
- Post-transcriptional gene silencing in controlling viruses of the Tomato yellow leaf curl virus complex.
- Authors: Abhary MK, Anfoka GH, Nakhla MK, Maxwell DP
- Issue date: 2006 Dec
- CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion.
- Authors: Ali Z, Ali S, Tashkandi M, Zaidi SS, Mahfouz MM
- Issue date: 2016 May 26
- Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA.
- Authors: Leibman D, Prakash S, Wolf D, Zelcer A, Anfoka G, Haviv S, Brumin M, Gaba V, Arazi T, Lapidot M, Gal-On A
- Issue date: 2015 Nov