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dc.contributor.authorAbdallah, Abdallah
dc.contributor.authorHill-Cawthorne, Grant A.
dc.contributor.authorOtto, Thomas D.
dc.contributor.authorColl, Francesc
dc.contributor.authorGuerra-Assunção, José Afonso
dc.contributor.authorGao, Ge
dc.contributor.authorNaeem, Raeece
dc.contributor.authorAnsari, Hifzur Rahman
dc.contributor.authorMalas, Tareq Majed Yasin
dc.contributor.authorAdroub, Sabir
dc.contributor.authorVerboom, Theo
dc.contributor.authorUmmels, Roy
dc.contributor.authorZhang, Huoming
dc.contributor.authorPanigrahi, Aswini Kumar
dc.contributor.authorMcNerney, Ruth
dc.contributor.authorBrosch, Roland
dc.contributor.authorClark, Taane G.
dc.contributor.authorBehr, Marcel A.
dc.contributor.authorBitter, Wilbert
dc.contributor.authorPain, Arnab
dc.date.accessioned2015-11-05T06:30:39Z
dc.date.available2015-11-05T06:30:39Z
dc.date.issued2015-10-21
dc.identifier.citationGenomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations 2015, 5:15443 Scientific Reports
dc.identifier.issn2045-2322
dc.identifier.pmid26487098
dc.identifier.doi10.1038/srep15443
dc.identifier.urihttp://hdl.handle.net/10754/581771
dc.description.abstractAlthough Bacillus Calmette-Guérin (BCG) vaccines against tuberculosis have been available for more than 90 years, their effectiveness has been hindered by variable protective efficacy and a lack of lasting memory responses. One factor contributing to this variability may be the diversity of the BCG strains that are used around the world, in part from genomic changes accumulated during vaccine production and their resulting differences in gene expression. We have compared the genomes and transcriptomes of a global collection of fourteen of the most widely used BCG strains at single base-pair resolution. We have also used quantitative proteomics to identify key differences in expression of proteins across five representative BCG strains of the four tandem duplication (DU) groups. We provide a comprehensive map of single nucleotide polymorphisms (SNPs), copy number variation and insertions and deletions (indels) across fourteen BCG strains. Genome-wide SNP characterization allowed the construction of a new and robust phylogenic genealogy of BCG strains. Transcriptional and proteomic profiling revealed a metabolic remodeling in BCG strains that may be reflected by altered immunogenicity and possibly vaccine efficacy. Together, these integrated-omic data represent the most comprehensive catalogue of genetic variation across a global collection of BCG strains.
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.urlhttp://www.nature.com/articles/srep15443
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.titleGenomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Core Lab
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer Science Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentPathogen Genomics Laboratory
dc.identifier.journalScientific Reports
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionMarie Bashir Institute for Infectious Diseases and Biosecurity and School and Public Health, University of Sydney, NSW 2006, Australia
dc.contributor.institutionPathogen Genomics, The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
dc.contributor.institutionFaculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personAbdallah, Abdallah
kaust.personHill-Cawthorne, Grant A.
kaust.personGao, Ge
kaust.personNaeem, Raeece
kaust.personAnsari, Hifzur Rahman
kaust.personMalas, Tareq Majed Yasin
kaust.personAdroub, Sabir
kaust.personZhang, Huoming
kaust.personPanigrahi, Aswini Kumar
kaust.personPain, Arnab
refterms.dateFOA2018-06-14T09:22:57Z
dc.date.published-online2015-10-21
dc.date.published-print2015-12


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