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    Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations

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    Type
    Article
    Authors
    Abdallah, Abdallah
    Hill-Cawthorne, Grant A. cc
    Otto, Thomas D.
    Coll, Francesc cc
    Guerra-Assunção, José Afonso
    Gao, Ge
    Naeem, Raeece cc
    Ansari, Hifzur Rahman cc
    Malas, Tareq Majed Yasin cc
    Adroub, Sabir
    Verboom, Theo
    Ummels, Roy
    Zhang, Huoming cc
    Panigrahi, Aswini Kumar
    McNerney, Ruth cc
    Brosch, Roland cc
    Clark, Taane G. cc
    Behr, Marcel A.
    Bitter, Wilbert cc
    Pain, Arnab cc
    KAUST Department
    Bioscience Core Lab
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    Computer Science Program
    Pathogen Genomics Group
    Computational Bioscience Research Center (CBRC)
    Date
    2015-10-21
    Permanent link to this record
    http://hdl.handle.net/10754/581771
    
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    Abstract
    Although Bacillus Calmette-Guérin (BCG) vaccines against tuberculosis have been available for more than 90 years, their effectiveness has been hindered by variable protective efficacy and a lack of lasting memory responses. One factor contributing to this variability may be the diversity of the BCG strains that are used around the world, in part from genomic changes accumulated during vaccine production and their resulting differences in gene expression. We have compared the genomes and transcriptomes of a global collection of fourteen of the most widely used BCG strains at single base-pair resolution. We have also used quantitative proteomics to identify key differences in expression of proteins across five representative BCG strains of the four tandem duplication (DU) groups. We provide a comprehensive map of single nucleotide polymorphisms (SNPs), copy number variation and insertions and deletions (indels) across fourteen BCG strains. Genome-wide SNP characterization allowed the construction of a new and robust phylogenic genealogy of BCG strains. Transcriptional and proteomic profiling revealed a metabolic remodeling in BCG strains that may be reflected by altered immunogenicity and possibly vaccine efficacy. Together, these integrated-omic data represent the most comprehensive catalogue of genetic variation across a global collection of BCG strains.
    Citation
    Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations 2015, 5:15443 Scientific Reports
    Publisher
    Nature Publishing Group
    Journal
    Scientific Reports
    ISSN
    2045-2322
    DOI
    10.1038/srep15443
    PubMed ID
    26487098
    Additional Links
    http://www.nature.com/articles/srep15443
    ae974a485f413a2113503eed53cd6c53
    10.1038/srep15443
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Computer Science Program; Computational Bioscience Research Center (CBRC); Bioscience Core Lab; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

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