Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations
Type
ArticleAuthors
Abdallah, AbdallahHill-Cawthorne, Grant A.

Otto, Thomas D.
Coll, Francesc

Guerra-Assunção, José Afonso
Gao, Ge
Naeem, Raeece

Ansari, Hifzur Rahman

Malas, Tareq Majed Yasin

Adroub, Sabir
Verboom, Theo
Ummels, Roy
Zhang, Huoming

Panigrahi, Aswini Kumar
McNerney, Ruth

Brosch, Roland

Clark, Taane G.

Behr, Marcel A.
Bitter, Wilbert

Pain, Arnab

KAUST Department
Bioscience Core LabBiological and Environmental Sciences and Engineering (BESE) Division
Bioscience Program
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Computer Science Program
Pathogen Genomics Group
Computational Bioscience Research Center (CBRC)
Date
2015-10-21Permanent link to this record
http://hdl.handle.net/10754/581771
Metadata
Show full item recordAbstract
Although Bacillus Calmette-Guérin (BCG) vaccines against tuberculosis have been available for more than 90 years, their effectiveness has been hindered by variable protective efficacy and a lack of lasting memory responses. One factor contributing to this variability may be the diversity of the BCG strains that are used around the world, in part from genomic changes accumulated during vaccine production and their resulting differences in gene expression. We have compared the genomes and transcriptomes of a global collection of fourteen of the most widely used BCG strains at single base-pair resolution. We have also used quantitative proteomics to identify key differences in expression of proteins across five representative BCG strains of the four tandem duplication (DU) groups. We provide a comprehensive map of single nucleotide polymorphisms (SNPs), copy number variation and insertions and deletions (indels) across fourteen BCG strains. Genome-wide SNP characterization allowed the construction of a new and robust phylogenic genealogy of BCG strains. Transcriptional and proteomic profiling revealed a metabolic remodeling in BCG strains that may be reflected by altered immunogenicity and possibly vaccine efficacy. Together, these integrated-omic data represent the most comprehensive catalogue of genetic variation across a global collection of BCG strains.Citation
Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations 2015, 5:15443 Scientific ReportsPublisher
Nature Publishing GroupJournal
Scientific ReportsISSN
2045-2322PubMed ID
26487098Additional Links
http://www.nature.com/articles/srep15443ae974a485f413a2113503eed53cd6c53
10.1038/srep15443
Scopus Count
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