Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations
Hill-Cawthorne, Grant A.
Otto, Thomas D.
Guerra-Assunção, José Afonso
Ansari, Hifzur Rahman
Malas, Tareq Majed Yasin
Panigrahi, Aswini Kumar
Clark, Taane G.
Behr, Marcel A.
KAUST DepartmentBioscience Core Lab
Biological and Environmental Sciences and Engineering (BESE) Division
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Computer Science Program
Pathogen Genomics Group
Computational Bioscience Research Center (CBRC)
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AbstractAlthough Bacillus Calmette-Guérin (BCG) vaccines against tuberculosis have been available for more than 90 years, their effectiveness has been hindered by variable protective efficacy and a lack of lasting memory responses. One factor contributing to this variability may be the diversity of the BCG strains that are used around the world, in part from genomic changes accumulated during vaccine production and their resulting differences in gene expression. We have compared the genomes and transcriptomes of a global collection of fourteen of the most widely used BCG strains at single base-pair resolution. We have also used quantitative proteomics to identify key differences in expression of proteins across five representative BCG strains of the four tandem duplication (DU) groups. We provide a comprehensive map of single nucleotide polymorphisms (SNPs), copy number variation and insertions and deletions (indels) across fourteen BCG strains. Genome-wide SNP characterization allowed the construction of a new and robust phylogenic genealogy of BCG strains. Transcriptional and proteomic profiling revealed a metabolic remodeling in BCG strains that may be reflected by altered immunogenicity and possibly vaccine efficacy. Together, these integrated-omic data represent the most comprehensive catalogue of genetic variation across a global collection of BCG strains.
CitationGenomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations 2015, 5:15443 Scientific Reports
PublisherNature Publishing Group
- [Efficacy and safety of vaccines against tuberculosis in the relation to genetic variability of Mycobacterium bovis BCG strains].
- Authors: Prygiel M, Janaszek-Seydlitz W, Bucholc B
- Issue date: 2011
- A comprehensive survey of single nucleotide polymorphisms (SNPs) across Mycobacterium bovis strains and M. bovis BCG vaccine strains refines the genealogy and defines a minimal set of SNPs that separate virulent M. bovis strains and M. bovis BCG strains.
- Authors: Garcia Pelayo MC, Uplekar S, Keniry A, Mendoza Lopez P, Garnier T, Nunez Garcia J, Boschiroli L, Zhou X, Parkhill J, Smith N, Hewinson RG, Cole ST, Gordon SV
- Issue date: 2009 May
- Novel genome polymorphisms in BCG vaccine strains and impact on efficacy.
- Authors: Leung AS, Tran V, Wu Z, Yu X, Alexander DC, Gao GF, Zhu B, Liu J
- Issue date: 2008 Sep 15
- Genome plasticity of BCG and impact on vaccine efficacy.
- Authors: Brosch R, Gordon SV, Garnier T, Eiglmeier K, Frigui W, Valenti P, Dos Santos S, Duthoy S, Lacroix C, Garcia-Pelayo C, Inwald JK, Golby P, Garcia JN, Hewinson RG, Behr MA, Quail MA, Churcher C, Barrell BG, Parkhill J, Cole ST
- Issue date: 2007 Mar 27
- Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis.
- Authors: Mahairas GG, Sabo PJ, Hickey MJ, Singh DC, Stover CK
- Issue date: 1996 Mar