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    A simple system for in-droplet incubation and quantification of agglutination assays

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    Description:
    A simple system for in-droplet incubation and quantification of agglutination assays
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    Type
    Conference Paper
    Authors
    Castro, David
    Kodzius, Rimantas cc
    Foulds, Ian G.
    KAUST Department
    Computational Bioscience Research Center (CBRC)
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    Date
    2013-10-28
    Permanent link to this record
    http://hdl.handle.net/10754/580854
    
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    Abstract
    This work reports on a simple system for quantitative sensing of a target analyte based on agglutination in micro-channels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (~2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.
    Publisher
    Royal Society of Chemistry
    Journal
    MicroTAS conference proceedings
    Conference/Event name
    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
    ISBN
    978-0-9798064-6-9
    Additional Links
    http://www.microtas2013.org/
    Collections
    Conference Papers; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division

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