NMR and MALDI-TOF MS based characterization of exopolysaccharides in anaerobic microbial aggregates from full-scale reactors
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Bioscience Core Lab
Computational Bioscience Research Center (CBRC)
Environmental Science and Engineering Program
Imaging and Characterization Core Lab
Nanofabrication Core Lab
Water Desalination and Reuse Research Center (WDRC)
Online Publication Date2015-09-22
Print Publication Date2015-11
Permanent link to this recordhttp://hdl.handle.net/10754/579487
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AbstractAnaerobic granular sludge is composed of multispecies microbial aggregates embedded in a matrix of extracellular polymeric substances (EPS). Here we characterized the chemical fingerprint of the polysaccharide fraction of EPS in anaerobic granules obtained from full-scale reactors treating different types of wastewater. Nuclear magnetic resonance (NMR) signals of the polysaccharide region from the granules were very complex, likely as a result of the diverse microbial population in the granules. Using nonmetric multidimensional scaling (NMDS), the 1H NMR signals of reference polysaccharides (gellan, xanthan, alginate) and those of the anaerobic granules revealed that there were similarities between the polysaccharides extracted from granules and the reference polysaccharide alginate. Further analysis of the exopolysaccharides from anaerobic granules, and reference polysaccharides using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) revealed that exopolysaccharides from two of the anaerobic granular sludges studied exhibited spectra similar to that of alginate. The presence of sequences related to the synthesis of alginate was confirmed in the metagenomes of the granules. Collectively these results suggest that alginate-like exopolysaccharides are constituents of the EPS matrix in anaerobic granular sludge treating different industrial wastewater. This finding expands the engineered environments where alginate has been found as EPS constituent of microbial aggregates.
CitationNMR and MALDI-TOF MS based characterization of exopolysaccharides in anaerobic microbial aggregates from full-scale reactors 2015, 5:14316 Scientific Reports
CollectionsNanofabrication Core Lab; Articles; Biological and Environmental Science and Engineering (BESE) Division; Environmental Science and Engineering Program; Imaging and Characterization Core Lab; Computational Bioscience Research Center (CBRC); Water Desalination and Reuse Research Center (WDRC); Bioscience Core Lab
- Aerobic sludge granulation: a tale of two polysaccharides?
- Authors: Seviour T, Yuan Z, van Loosdrecht MC, Lin Y
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- Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols.
- Authors: D'Abzac P, Bordas F, Van Hullebusch E, Lens PN, Guibaud G
- Issue date: 2010 Feb
- Extracellular polymeric substances (EPS) in upflow anaerobic sludge blanket (UASB) reactors operated under high salinity conditions.
- Authors: Ismail SB, de La Parra CJ, Temmink H, van Lier JB
- Issue date: 2010 Mar
- Gel-forming exopolysaccharides explain basic differences between structures of aerobic sludge granules and floccular sludges.
- Authors: Seviour T, Pijuan M, Nicholson T, Keller J, Yuan Z
- Issue date: 2009 Oct
- Characterization of alginate-like exopolysaccharides isolated from aerobic granular sludge in pilot-plant.
- Authors: Lin Y, de Kreuk M, van Loosdrecht MC, Adin A
- Issue date: 2010 Jun