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dc.contributor.authorDaniels, Camille Arian
dc.contributor.authorBaumgarten, Sebastian
dc.contributor.authorYum, Lauren
dc.contributor.authorMichell, Craig
dc.contributor.authorBayer, Till
dc.contributor.authorArif, Chatchanit
dc.contributor.authorRoder, Cornelia
dc.contributor.authorWeil, Ernesto
dc.contributor.authorVoolstra, Christian R.
dc.date.accessioned2015-09-15T12:39:48Z
dc.date.available2015-09-15T12:39:48Z
dc.date.issued2015-09-11
dc.identifier.citationMetatranscriptome analysis of the reef-building coral Orbicella faveolata indicates holobiont response to coral disease 2015, 2 Frontiers in Marine Science
dc.identifier.issn2296-7745
dc.identifier.doi10.3389/fmars.2015.00062
dc.identifier.urihttp://hdl.handle.net/10754/577334
dc.description.abstractWhite Plague Disease (WPD) is implicated in coral reef decline in the Caribbean and is characterized by microbial community shifts in coral mucus and tissue. Studies thus far have focused on assessing microbial communities or the identification of specific pathogens, yet few have addressed holobiont response across metaorganism compartments in coral disease. Here, we report on the first metatranscriptomic assessment of the coral host, algal symbiont, and microbial compartment in order to survey holobiont structure and function in healthy and diseased samples from Orbicella faveolata collected at reef sites off Puerto Rico. Our data indicate holobiont-wide as well as compartment-specific responses to WPD. Gene expression changes in the diseased coral host involved proteins playing a role in innate immunity, cytoskeletal integrity, cell adhesion, oxidative stress, chemical defense, and retroelements. In contrast, the algal symbiont showed comparatively few expression changes, but of large magnitude, of genes related to stress, photosynthesis, and metal transport. Concordant with the coral host response, the bacterial compartment showed increased abundance of heat shock proteins, genes related to oxidative stress, DNA repair, and potential retroelement activity. Importantly, analysis of the expressed bacterial gene functions establishes the participation of multiple bacterial families in WPD pathogenesis and also suggests a possible involvement of viruses and/or phages in structuring the bacterial assemblage. In this study, we implement an experimental approach to partition the coral holobiont and resolve compartment- and taxa-specific responses in order to understand metaorganism function in coral disease.
dc.language.isoen
dc.publisherFrontiers Media SA
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmars.2015.00062
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. http://creativecommons.org/licenses/by/4.0/
dc.subjectcoral reef
dc.subjectcoral disease
dc.subjectmetatranscriptomics
dc.subjectSymbiodinium
dc.subjectmetaorganism
dc.titleMetatranscriptome analysis of the reef-building coral Orbicella faveolata indicates holobiont response to coral disease
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalFrontiers in Marine Science
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionGEOMAR Department: Evolutionary Ecology of Marine Fishes, GEOMAR Helmholtz Centre for Ocean Research, Kiel, Germany
dc.contributor.institutionDepartment of Marine Sciences, University of Puerto Rico, Mayaguez, Puerto Rico
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personDaniels, Camille Arian
kaust.personBaumgarten, Sebastian
kaust.personYum, Lauren
kaust.personMichell, Craig
kaust.personBayer, Till
kaust.personArif, Chatchanit
kaust.personRoder, Cornelia
kaust.personVoolstra, Christian R.
dc.relation.issupplementedbybioproject:PRJNA289876
refterms.dateFOA2018-06-13T12:17:36Z
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Bioproject]</i> <br/> Title: Orbicella faveolata WPD holotranscriptome sequencingPublication Date: 2015-07-14. bioproject: <a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA289876" >PRJNA289876</a> Handle: <a href="http://hdl.handle.net/10754/666546" >10754/666546</a></a></li></ul>


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