Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA
KAUST DepartmentBioscience Core Lab
Online Publication Date2011-12-12
Print Publication Date2012-06
Permanent link to this recordhttp://hdl.handle.net/10754/577061
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AbstractFull-length cDNA (435 bp) of the interleukin-3(IL-3) gene of the Indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL-3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. Structural homology modelling of buffalo IL-3 protein with human IL-3 showed the presence of five helical structures.
CitationThennarasu, S., Harishankar, M., & Dhinakar Raj, G. (2011). Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA. International Journal of Immunogenetics, 39(3), 203–206. doi:10.1111/j.1744-313x.2011.01075.x
SponsorsThe authors thank the Department of Biotechnology (DBT), Government of India, for financial assistance to this Project. ST and MHS were SRFs who worked in this project.