Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA
Type
ArticleKAUST Department
Bioscience Core LabDate
2011-12-12Online Publication Date
2011-12-12Print Publication Date
2012-06Permanent link to this record
http://hdl.handle.net/10754/577061
Metadata
Show full item recordAbstract
Full-length cDNA (435 bp) of the interleukin-3(IL-3) gene of the Indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL-3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. Structural homology modelling of buffalo IL-3 protein with human IL-3 showed the presence of five helical structures.Citation
Thennarasu, S., Harishankar, M., & Dhinakar Raj, G. (2011). Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA. International Journal of Immunogenetics, 39(3), 203–206. doi:10.1111/j.1744-313x.2011.01075.xSponsors
The authors thank the Department of Biotechnology (DBT), Government of India, for financial assistance to this Project. ST and MHS were SRFs who worked in this project.Publisher
Wileyae974a485f413a2113503eed53cd6c53
10.1111/j.1744-313X.2011.01075.x