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dc.contributor.authorPapsdorf, Katharina
dc.contributor.authorKaiser, Christoph J. O.
dc.contributor.authorDrazic, Adrian
dc.contributor.authorGrötzinger, Stefan W.
dc.contributor.authorHaeßner, Carmen
dc.contributor.authorEisenreich, Wolfgang
dc.contributor.authorRichter, Klaus
dc.date.accessioned2015-09-09T06:14:42Z
dc.date.available2015-09-09T06:14:42Z
dc.date.issued2015-09-03
dc.identifier.citationPolyglutamine toxicity in yeast induces metabolic alterations and mitochondrial defects 2015, 16 (1) BMC Genomics
dc.identifier.issn1471-2164
dc.identifier.pmid26335097
dc.identifier.doi10.1186/s12864-015-1831-7
dc.identifier.urihttp://hdl.handle.net/10754/576984
dc.description.abstractBackground Protein aggregation and its pathological effects are the major cause of several neurodegenerative diseases. In Huntington’s disease an elongated stretch of polyglutamines within the protein Huntingtin leads to increased aggregation propensity. This induces cellular defects, culminating in neuronal loss, but the connection between aggregation and toxicity remains to be established. Results To uncover cellular pathways relevant for intoxication we used genome-wide analyses in a yeast model system and identify fourteen genes that, if deleted, result in higher polyglutamine toxicity. Several of these genes, like UGO1, ATP15 and NFU1 encode mitochondrial proteins, implying that a challenged mitochondrial system may become dysfunctional during polyglutamine intoxication. We further employed microarrays to decipher the transcriptional response upon polyglutamine intoxication, which exposes an upregulation of genes involved in sulfur and iron metabolism and mitochondrial Fe-S cluster formation. Indeed, we find that in vivo iron concentrations are misbalanced and observe a reduction in the activity of the prominent Fe-S cluster containing protein aconitase. Like in other yeast strains with impaired mitochondria, non-fermentative growth is impossible after intoxication with the polyglutamine protein. NMR-based metabolic analyses reveal that mitochondrial metabolism is reduced, leading to accumulation of metabolic intermediates in polyglutamine-intoxicated cells. Conclusion These data show that damages to the mitochondrial system occur in polyglutamine intoxicated yeast cells and suggest an intricate connection between polyglutamine-induced toxicity, mitochondrial functionality and iron homeostasis in this model system.
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.urlhttp://www.biomedcentral.com/1471-2164/16/662
dc.rightsOpen AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.subjectPolyglutamine
dc.subjectIron-sulfur cluster
dc.subjectMitochondria
dc.subjectNeurodegenerative disease
dc.titlePolyglutamine toxicity in yeast induces metabolic alterations and mitochondrial defects
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentBiological and Organometallic Catalysis Laboratories
dc.identifier.journalBMC Genomics
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment Chemie, Lehrstuhl für Biotechnologie, Technische Universität München, Lichtenbergstraße 4, Garching, 85748, Germany
dc.contributor.institutionDepartment Chemie, Fachgebiet Elektronenmikroskopie, Technische Universität München, Lichtenbergstraße 4, Garching, 85748, Germany
dc.contributor.institutionDepartment Chemie, Fachgebiet Anorganische Chemie, Technische Universität München, Lichtenbergstraße 4, Garching, 85748, Germany
dc.contributor.institutionDepartment Chemie, Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstraße 4, Garching, 85748, Germany
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personGrötzinger, Stefan W.
dc.relation.issupplementedbyDOI:10.6084/m9.figshare.c.3618170
refterms.dateFOA2018-06-14T05:00:05Z
display.relations<b> Is Supplemented By:</b> <br/> <ul><li><i>[Dataset]</i> <br/> Papsdorf, K., Kaiser, C., Drazic, A., Grötzinger, S., HaeßNer, C., Eisenreich, W., & Richter, K. (2015). Polyglutamine toxicity in yeast induces metabolic alterations and mitochondrial defects. Figshare. https://doi.org/10.6084/m9.figshare.c.3618170. DOI: <a href="https://doi.org/10.6084/m9.figshare.c.3618170">10.6084/m9.figshare.c.3618170</a> HANDLE: <a href="http://hdl.handle.net/10754/624133">10754/624133</a></li></ul>
dc.date.published-online2015-09-03
dc.date.published-print2015-12


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