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dc.contributor.authorMarini, Monica
dc.contributor.authorFalqui, Andrea
dc.contributor.authorMoretti, Manola
dc.contributor.authorLimongi, Tania
dc.contributor.authorAllione, Marco
dc.contributor.authorGenovese, Alessandro
dc.contributor.authorLopatin, Sergei
dc.contributor.authorTirinato, Luca
dc.contributor.authorDas, Gobind
dc.contributor.authorTorre, Bruno
dc.contributor.authorGiugni, Andrea
dc.contributor.authorGentile, Francesco
dc.contributor.authorCandeloro, Patrizio
dc.contributor.authorDi Fabrizio, Enzo M.
dc.date.accessioned2015-09-03T12:50:20Z
dc.date.available2015-09-03T12:50:20Z
dc.date.issued2015-08-28
dc.identifier.citationThe structure of DNA by direct imaging 2015, 1 (7):e1500734 Science Advances
dc.identifier.issn2375-2548
dc.identifier.pmid26601243
dc.identifier.doi10.1126/sciadv.1500734
dc.identifier.urihttp://hdl.handle.net/10754/576450
dc.description.abstractThe structure of DNA was determined in 1953 by x-ray fiber diffraction. Several attempts have been made to obtain a direct image of DNA with alternative techniques. The direct image is intended to allow a quantitative evaluation of all relevant characteristic lengths present in a molecule. A direct image of DNA, which is different from diffraction in the reciprocal space, is difficult to obtain for two main reasons: the intrinsic very low contrast of the elements that form the molecule and the difficulty of preparing the sample while preserving its pristine shape and size. We show that through a preparation procedure compatible with the DNA physiological conditions, a direct image of a single suspended DNA molecule can be obtained. In the image, all relevant lengths of A-form DNA are measurable. A high-resolution transmission electron microscope that operates at 80 keV with an ultimate resolution of 1.5 Å was used for this experiment. Direct imaging of a single molecule can be used as a method to address biological problems that require knowledge at the single-molecule level, given that the average information obtained by x-ray diffraction of crystals or fibers is not sufficient for detailed structure determination, or when crystals cannot be obtained from biological molecules or are not sufficient in understanding multiple protein configurations.
dc.language.isoen
dc.publisherAmerican Association for the Advancement of Science (AAAS)
dc.relation.urlhttp://advances.sciencemag.org/cgi/doi/10.1126/sciadv.1500734
dc.rights2015 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 10.1126/sciadv.1500734
dc.titleThe structure of DNA by direct imaging
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentElectron Microscopy
dc.contributor.departmentImaging and Characterization Core Lab
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentNABLA Lab
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.contributor.departmentSMILEs Lab
dc.identifier.journalScience Advances
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionBio Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia, Viale Europa, Germaneto, 88100 Catanzaro, Italy
dc.contributor.institutionDepartment of Electrical Engineering and Information Technology, University of Naples, 80125 Naples, Italy
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personMarini, Monica
kaust.personFalqui, Andrea
kaust.personMoretti, Manola
kaust.personLimongi, Tania
kaust.personAllione, Marco
kaust.personGenovese, Alessandro
kaust.personLopatin, Sergei
kaust.personTirinato, Luca
kaust.personDas, Gobind
kaust.personTorre, Bruno
kaust.personGiugni, Andrea
kaust.personDi Fabrizio, Enzo M.
refterms.dateFOA2018-06-13T10:26:44Z
dc.date.published-online2015-08-28
dc.date.published-print2015-08-28


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