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    The structure of DNA by direct imaging

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    Type
    Article
    Authors
    Marini, Monica cc
    Falqui, Andrea cc
    Moretti, Manola
    Limongi, Tania
    Allione, Marco cc
    Genovese, Alessandro
    Lopatin, Sergei cc
    Tirinato, Luca cc
    Das, Gobind cc
    Torre, Bruno
    Giugni, Andrea
    Gentile, Francesco
    Candeloro, Patrizio cc
    Di Fabrizio, Enzo M. cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    Electron Microscopy
    Imaging and Characterization Core Lab
    Material Science and Engineering Program
    NABLA Lab
    Physical Science and Engineering (PSE) Division
    SMILEs Lab
    Date
    2015-08-28
    Online Publication Date
    2015-08-28
    Print Publication Date
    2015-08-28
    Permanent link to this record
    http://hdl.handle.net/10754/576450
    
    Metadata
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    Abstract
    The structure of DNA was determined in 1953 by x-ray fiber diffraction. Several attempts have been made to obtain a direct image of DNA with alternative techniques. The direct image is intended to allow a quantitative evaluation of all relevant characteristic lengths present in a molecule. A direct image of DNA, which is different from diffraction in the reciprocal space, is difficult to obtain for two main reasons: the intrinsic very low contrast of the elements that form the molecule and the difficulty of preparing the sample while preserving its pristine shape and size. We show that through a preparation procedure compatible with the DNA physiological conditions, a direct image of a single suspended DNA molecule can be obtained. In the image, all relevant lengths of A-form DNA are measurable. A high-resolution transmission electron microscope that operates at 80 keV with an ultimate resolution of 1.5 Å was used for this experiment. Direct imaging of a single molecule can be used as a method to address biological problems that require knowledge at the single-molecule level, given that the average information obtained by x-ray diffraction of crystals or fibers is not sufficient for detailed structure determination, or when crystals cannot be obtained from biological molecules or are not sufficient in understanding multiple protein configurations.
    Citation
    The structure of DNA by direct imaging 2015, 1 (7):e1500734 Science Advances
    Publisher
    American Association for the Advancement of Science (AAAS)
    Journal
    Science Advances
    DOI
    10.1126/sciadv.1500734
    PubMed ID
    26601243
    Additional Links
    http://advances.sciencemag.org/cgi/doi/10.1126/sciadv.1500734
    ae974a485f413a2113503eed53cd6c53
    10.1126/sciadv.1500734
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Imaging and Characterization Core Lab; Physical Science and Engineering (PSE) Division; Material Science and Engineering Program; Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division

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