Selective phosphorylation during early macrophage differentiation
KAUST DepartmentBioscience Core Lab
Biological and Environmental Sciences and Engineering (BESE) Division
Applied Mathematics and Computational Science Program
Online Publication Date2015-09-15
Print Publication Date2015-11
Permanent link to this recordhttp://hdl.handle.net/10754/576076
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AbstractThe differentiation of macrophages from monocytes is a tightly controlled and complex biological process. Although numerous studies have been conducted using biochemical approaches or global gene/gene profiling, the mechanisms of the early stages of differentiation remain unclear. Here we used SILAC-based quantitative proteomics approach to perform temporal phosphoproteome profiling of early macrophage differentiation. We identified a large set of phosphoproteins and grouped them as PMA-regulated and non-regulated phosphoproteins in the early stages of differentiation. Further analysis of the PMA-regulated phosphoproteins revealed that transcriptional suppression, cytoskeletal reorganization and cell adhesion were among the most significantly activated pathways. Some key involved regulators of these pathways are mTOR, MYB, STAT1 and CTNNB. Moreover, we were able to classify the roles and activities of several transcriptional factors during different differentiation stages and found that E2F is likely to be an important regulator during the relatively late stages of differentiation. This study provides the first comprehensive picture of the dynamic phosphoproteome during myeloid cells differentiation, and identifies potential molecular targets in leukemic cells.
CitationSelective phosphorylation during early macrophage differentiation 2015:n/a PROTEOMICS
- [Phosphoprotein affinity profiling--a novel method for screening regulators of lipopolysaccharide signaling pathway].
- Authors: Zou ZP, Li YS, Chen J, Jiang Y
- Issue date: 2007 Jun
- Phosphoproteomic analysis of human mesenchymal stromal cells during osteogenic differentiation.
- Authors: Lo T, Tsai CF, Shih YR, Wang YT, Lu SC, Sung TY, Hsu WL, Chen YJ, Lee OK
- Issue date: 2012 Feb 3
- [Ananlysis of phosphoproteins and signalling pathways by quantitative proteomics].
- Authors: Pjechová M, Hernychova L, Tomašec P, Wilkinson GW, Vojtěšek B
- Issue date: 2014
- Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.
- Authors: Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M
- Issue date: 2010 Jan 12
- Comprehensive phosphoproteome analysis of INS-1 pancreatic β-cells using various digestion strategies coupled with liquid chromatography-tandem mass spectrometry.
- Authors: Han D, Moon S, Kim Y, Ho WK, Kim K, Kang Y, Jun H, Kim Y
- Issue date: 2012 Apr 6