Biophysical analysis of a lethal laminin alpha-1 mutation reveals altered self-interaction
Type
ArticleAuthors
Patel, Trushar R.
Nikodemus, Denise
Besong, Tabot M.D.

Reuten, Raphael
Meier, Markus
Harding, Stephen E.
Winzor, Donald J.
Koch, Manuel
Stetefeld, Jörg
KAUST Department
Functional Nanomaterials Lab (FuNL)KAUST Solar Center (KSC)
Date
2015-07-26Online Publication Date
2015-07-26Print Publication Date
2016-01Permanent link to this record
http://hdl.handle.net/10754/575931
Metadata
Show full item recordAbstract
Laminins are key basement membrane molecules that influence several biological activities and are linked to a number of diseases. They are secreted as heterotrimeric proteins consisting of one α, one β, and one γ chain, followed by their assembly into a polymer-like sheet at the basement membrane. Using sedimentation velocity, dynamic light scattering, and surface plasmon resonance experiments, we studied self-association of three laminin (LM) N-terminal fragments α-1 (hLM α-1 N), α-5 (hLM α-5 N) and β-3 (hLM β-3 N) originating from the short arms of the human laminin αβγ heterotrimer. Corresponding studies of the hLM α-1 N C49S mutant, equivalent to the larval lethal C56S mutant in zebrafish, have shown that this mutation causes enhanced self-association behavior, an observation that provides a plausible explanation for the inability of laminin bearing this mutation to fulfill functional roles in vivo, and hence for the deleterious pathological consequences of the mutation on lens function.Citation
Patel TR, Nikodemus D, Besong TMD, Reuten R, Meier M, et al. (2015) Biophysical analysis of a lethal laminin alpha-1 mutation reveals altered self-interaction. Matrix Biology. Available: http://dx.doi.org/10.1016/j.matbio.2015.06.005.Sponsors
TRP was the recipient of a Canadian Institutes of Health Research postdoctoral fellowship and is currently supported by the Marie Skłodowska-Curie Fellowship. JS holds a Canada Research Chair in Structure Biology. This investigation was supported in part by the Multiple Sclerosis Society of Canada and by the Canadian Institute of Health Research (RPA-109759).Publisher
Elsevier BVJournal
Matrix BiologyPubMed ID
26215696Additional Links
http://linkinghub.elsevier.com/retrieve/pii/S0945053X15001237ae974a485f413a2113503eed53cd6c53
10.1016/j.matbio.2015.06.005
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