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dc.contributor.authorBruggeman, Quentin
dc.contributor.authorPrunier, Florence
dc.contributor.authorMazubert, Christelle
dc.contributor.authorde Bont, Linda
dc.contributor.authorGarmier, Marie
dc.contributor.authorLugan, Raphaël
dc.contributor.authorBenhamed, Moussa
dc.contributor.authorBergounioux, Catherine
dc.contributor.authorRaynaud, Cécile
dc.contributor.authorDelarue, Marianne
dc.date.accessioned2015-08-24T08:35:04Z
dc.date.available2015-08-24T08:35:04Z
dc.date.issued2015-06-05
dc.identifier.issn1040-4651
dc.identifier.issn1532-298X
dc.identifier.pmid26048869
dc.identifier.doi10.1105/tpc.15.00068
dc.identifier.urihttp://hdl.handle.net/10754/575652
dc.description.abstractProgrammed cell death (PCD) is essential for several aspects of plant life, including development and stress responses. We recently identified the mips1 mutant of Arabidopsis thaliana, which is deficient for the enzyme catalyzing the limiting step of myo-inositol (MI) synthesis. One of the most striking features of mips1 is the light-dependent formation of lesions on leaves due to salicylic acid (SA)-dependent PCD. Here, we identified a suppressor of PCD by screening for mutations that abolish the mips1 cell death phenotype. Our screen identified the hxk1 mutant, mutated in the gene encoding the hexokinase1 (HXK1) enzyme that catalyzes sugar phosphorylation and acts as a genuine glucose sensor. We show that HXK1 is required for lesion formation in mips1 due to alterations in MI content, via SA-dependant signaling. Using two catalytically inactive HXK1 mutants, we also show that hexokinase catalytic activity is necessary for the establishment of lesions in mips1. Gas chromatography-mass spectrometry analyses revealed a restoration of the MI content in mips1 hxk1 that it is due to the activity of the MIPS2 isoform, while MIPS3 is not involved. Our work defines a pathway of HXK1-mediated cell death in plants and demonstrates that two MIPS enzymes act cooperatively under a particular metabolic status, highlighting a novel checkpoint of MI homeostasis in plants. © 2015 American Society of Plant Biologists. All rights reserved.
dc.publisherAmerican Society of Plant Biologists (ASPB)
dc.titleInvolvement of Arabidopsis Hexokinase1 in Cell Death Mediated by Myo -Inositol Accumulation
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentBioscience Program
dc.contributor.departmentChromatin and development Research Group
dc.identifier.journalThe Plant Cell
dc.contributor.institutionUniversité Paris-Sud, Institute of Plant Sciences Paris-Saclay IPS2 (Bâtiment 630), UMR CNRS-INRA 9213, Saclay Plant Sciences, 91405 Orsay, France
dc.contributor.institutionInstitut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 67084 Strasbourg Cedex, France
kaust.personBenhamed, Moussa
dc.date.published-online2015-06-05
dc.date.published-print2015-06


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