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    Involvement of Arabidopsis Hexokinase1 in Cell Death Mediated by Myo -Inositol Accumulation

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    Type
    Article
    Authors
    Bruggeman, Quentin
    Prunier, Florence
    Mazubert, Christelle
    De Bont, Linda
    Garmier, Marie
    Lugan, Raphaël
    Benhamed, Moussa cc
    Bergounioux, Catherine
    Raynaud, Cécile
    Delarue, Marianne
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Program
    Center for Desert Agriculture
    Chromatin and development Research Group
    Plant Science
    Plant Science Program
    Date
    2015-06-05
    Online Publication Date
    2015-06-05
    Print Publication Date
    2015-06
    Permanent link to this record
    http://hdl.handle.net/10754/575652
    
    Metadata
    Show full item record
    Abstract
    Programmed cell death (PCD) is essential for several aspects of plant life, including development and stress responses. We recently identified the mips1 mutant of Arabidopsis thaliana, which is deficient for the enzyme catalyzing the limiting step of myo-inositol (MI) synthesis. One of the most striking features of mips1 is the light-dependent formation of lesions on leaves due to salicylic acid (SA)-dependent PCD. Here, we identified a suppressor of PCD by screening for mutations that abolish the mips1 cell death phenotype. Our screen identified the hxk1 mutant, mutated in the gene encoding the hexokinase1 (HXK1) enzyme that catalyzes sugar phosphorylation and acts as a genuine glucose sensor. We show that HXK1 is required for lesion formation in mips1 due to alterations in MI content, via SA-dependant signaling. Using two catalytically inactive HXK1 mutants, we also show that hexokinase catalytic activity is necessary for the establishment of lesions in mips1. Gas chromatography-mass spectrometry analyses revealed a restoration of the MI content in mips1 hxk1 that it is due to the activity of the MIPS2 isoform, while MIPS3 is not involved. Our work defines a pathway of HXK1-mediated cell death in plants and demonstrates that two MIPS enzymes act cooperatively under a particular metabolic status, highlighting a novel checkpoint of MI homeostasis in plants. © 2015 American Society of Plant Biologists. All rights reserved.
    Citation
    Bruggeman, Q., Prunier, F., Mazubert, C., de Bont, L., Garmier, M., Lugan, R., … Delarue, M. (2015). Involvement of Arabidopsis Hexokinase1 in Cell Death Mediated by Myo-Inositol Accumulation. The Plant Cell, 27(6), 1801–1814. doi:10.1105/tpc.15.00068
    Publisher
    American Society of Plant Biologists (ASPB)
    Journal
    The Plant Cell
    DOI
    10.1105/tpc.15.00068
    PubMed ID
    26048869
    ae974a485f413a2113503eed53cd6c53
    10.1105/tpc.15.00068
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Center for Desert Agriculture

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