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dc.contributor.authorBagchi, Samik
dc.contributor.authorGarcia Tellez, Berenice
dc.contributor.authorRao, Hari Ananda
dc.contributor.authorLamendella, Regina
dc.contributor.authorSaikaly, Pascal
dc.date.accessioned2015-08-24T08:34:22Z
dc.date.available2015-08-24T08:34:22Z
dc.date.issued2014-10-19
dc.identifier.citationBagchi, S., Tellez, B. G., Rao, H. A., Lamendella, R., & Saikaly, P. E. (2014). Diversity and dynamics of dominant and rare bacterial taxa in replicate sequencing batch reactors operated under different solids retention time. Applied Microbiology and Biotechnology, 99(5), 2361–2370. doi:10.1007/s00253-014-6134-4
dc.identifier.issn01757598
dc.identifier.pmid25326778
dc.identifier.doi10.1007/s00253-014-6134-4
dc.identifier.urihttp://hdl.handle.net/10754/575620
dc.description.abstractIn this study, 16S rRNA gene pyrosequencing was applied in order to provide a better insight on the diversity and dynamics of total, dominant, and rare bacterial taxa in replicate lab-scale sequencing batch reactors (SBRs) operated at different solids retention time (SRT). Rank-abundance curves showed few dominant operational taxonomic units (OTUs) and a long tail of rare OTUs in all reactors. Results revealed that there was no detectable effect of SRT (2 vs. 10 days) on Shannon diversity index and OTU richness of both dominant and rare taxa. Nonmetric multidimensional scaling analysis showed that the total, dominant, and rare bacterial taxa were highly dynamic during the entire period of stable reactor performance. Also, the rare taxa were more dynamic than the dominant taxa despite expected low invasion rates because of the use of sterile synthetic media.
dc.description.sponsorshipThis research was supported by discretionary investigator funds (P.E.S.) at King Abdullah University of Science and Technology (KAUST). Special thanks are extended to Mohammed Alarawi at the Bioscience Core Laboratory at KAUST for their assistance in 16S rRNA gene pyrosequencing.
dc.publisherSpringer Nature
dc.subject16S rRNA gene pyrosequencing
dc.subjectActivated sludge
dc.subjectBacterial community dynamics
dc.subjectBacterial diversity
dc.subjectRare taxa
dc.titleDiversity and dynamics of dominant and rare bacterial taxa in replicate sequencing batch reactors operated under different solids retention time
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.identifier.journalApplied Microbiology and Biotechnology
dc.contributor.institutionVon Liebig Center for Science, Juniata College, 1700 Moore StreetHuntingdon, PA, United States
kaust.personBagchi, Samik
kaust.personGarcia Tellez, Berenice
kaust.personRao, Hari Ananda
kaust.personSaikaly, Pascal
kaust.acknowledged.supportUnitBioscience Core Laboratory
dc.date.published-online2014-10-19
dc.date.published-print2015-03


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