Marine Microorganisms as Source of Stereoselective Esterases and Ketoreductases: Kinetic Resolution of a Prostaglandin Intermediate
AuthorsDe Vitis, Valerio
Contente, Martina Letizia
Granato, Tiziana Mariarita
Borin, Sara S.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Extreme Systems Microbiology Lab
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AbstractA screening among bacterial strains isolated from water-brine interface of the deep hypersaline anoxic basins (DHABs) of the Eastern Mediterranean was carried out for the biocatalytical resolution of racemic propyl ester of anti-2-oxotricyclo[220.127.116.11]heptan-7-carboxylic acid (R,S)-1, a key intermediate for the synthesis of d-cloprostenol. Bacillus horneckiae 15A gave highly stereoselective reduction of (R,S)-1, whereas Halomonas aquamarina 9B enantioselectively hydrolysed (R,S)-1; in both cases, enantiomerically pure unreacted (R)-1 could be easily recovered and purified at molar conversion below 57–58 %, showing the potential of DHAB extremophile microbiome and marine-derived enzymes in stereoselective biocatalysis.
SponsorsF. Mapelli was supported by the Universita degli Studi di Milano, DeFENS, European Social Fund (FSE) and Regione Lombardia (Contract "Dote Ricerca"). This work was supported by the European Community FP7-KBBE.2012.3.2-02 project MACUMBA, grant agreement N. 311975.