Multiple and high-throughput droplet reactions via combination of microsampling technique and microfluidic chip
KAUST DepartmentKAUST-HKUST Micro/Nanofluidic Joint Laboratory
Environmental Science and Engineering Program
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AbstractMicrodroplets offer unique compartments for accommodating a large number of chemical and biological reactions in tiny volume with precise control. A major concern in droplet-based microfluidics is the difficulty to address droplets individually and achieve high throughput at the same time. Here, we have combined an improved cartridge sampling technique with a microfluidic chip to perform droplet screenings and aggressive reaction with minimal (nanoliter-scale) reagent consumption. The droplet composition, distance, volume (nanoliter to subnanoliter scale), number, and sequence could be precisely and digitally programmed through the improved sampling technique, while sample evaporation and cross-contamination are effectively eliminated. Our combined device provides a simple model to utilize multiple droplets for various reactions with low reagent consumption and high throughput. © 2012 American Chemical Society.
SponsorsThis publication is based on work partially supported by Award No. SA-C0040/UK-C0016, made by King Abdullah University of Science and Technology (KAUST), Hong Kong RGC grants HKUST 604710 and 605411.
PublisherAmerican Chemical Society (ACS)
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