Multiple and high-throughput droplet reactions via combination of microsampling technique and microfluidic chip
KAUST DepartmentEnvironmental Science and Engineering Program
KAUST-HKUST Micro/Nanofluidic Joint Laboratory
Water Desalination and Reuse Research Center (WDRC)
KAUST Grant NumberSA-C0040/UK-C0016
Online Publication Date2012-11
Print Publication Date2012-11-20
Permanent link to this recordhttp://hdl.handle.net/10754/575567
MetadataShow full item record
AbstractMicrodroplets offer unique compartments for accommodating a large number of chemical and biological reactions in tiny volume with precise control. A major concern in droplet-based microfluidics is the difficulty to address droplets individually and achieve high throughput at the same time. Here, we have combined an improved cartridge sampling technique with a microfluidic chip to perform droplet screenings and aggressive reaction with minimal (nanoliter-scale) reagent consumption. The droplet composition, distance, volume (nanoliter to subnanoliter scale), number, and sequence could be precisely and digitally programmed through the improved sampling technique, while sample evaporation and cross-contamination are effectively eliminated. Our combined device provides a simple model to utilize multiple droplets for various reactions with low reagent consumption and high throughput. © 2012 American Chemical Society.
CitationWu, J., Zhang, M., Li, X., & Wen, W. (2012). Multiple and High-Throughput Droplet Reactions via Combination of Microsampling Technique and Microfluidic Chip. Analytical Chemistry, 84(22), 9689–9693. doi:10.1021/ac302249h
SponsorsThis publication is based on work partially supported by Award No. SA-C0040/UK-C0016, made by King Abdullah University of Science and Technology (KAUST), Hong Kong RGC grants HKUST 604710 and 605411.
PublisherAmerican Chemical Society (ACS)
- Droplet-based microfluidic flow injection system with large-scale concentration gradient by a single nanoliter-scale injection for enzyme inhibition assay.
- Authors: Cai LF, Zhu Y, Du GS, Fang Q
- Issue date: 2012 Jan 3
- An integrated microfluidic chip for the analysis of biochemical reactions by MALDI mass spectrometry.
- Authors: Lee SH, Lee CS, Kim BG, Kim YK
- Issue date: 2008 Feb
- A fast and efficient microfluidic system for highly selective one-to-one droplet fusion.
- Authors: Mazutis L, Baret JC, Griffiths AD
- Issue date: 2009 Sep 21
- Controlled droplet microfluidic systems for multistep chemical and biological assays.
- Authors: Kaminski TS, Garstecki P
- Issue date: 2017 Oct 16
- Droplet microfluidics for high-throughput analysis of cells and particles.
- Authors: Zagnoni M, Cooper JM
- Issue date: 2011