Show simple item record

dc.contributor.authorZhang, Huoming
dc.contributor.authorZhao, Changqing
dc.contributor.authorLi, Xin
dc.contributor.authorZhu, Yi
dc.contributor.authorGan, Chee Sian
dc.contributor.authorWang, Yong
dc.contributor.authorRavasi, Timothy
dc.contributor.authorQian, Pei-Yuan
dc.contributor.authorWong, Siew Cheng
dc.contributor.authorSze, Siu Kwan
dc.date.accessioned2015-08-24T08:32:21Z
dc.date.available2015-08-24T08:32:21Z
dc.date.issued2010-07-02
dc.identifier.issn1615-9853
dc.identifier.doi10.1002/pmic.201000066
dc.identifier.urihttp://hdl.handle.net/10754/575541
dc.description.abstractHuman monocytes' exposure to low-level lipopolysaccharide (LPS) induces temporary monocytic insensitivity to subsequent LPS challenge. The underlying mechanism of this phenomenon could have important clinical utilities in preventing and/or treating severe infections. In this study, we used an iTRAQ-based quantitative proteomic approach to comprehensively characterize the membrane proteomes of monocytes before and after LPS exposure. We identified a total of 1651 proteins, of which 53.6% were membrane proteins. Ninety-four percent of the proteins were quantified and 255 proteins were shown to be tightly regulated by LPS. Subcellular location analysis revealed organelle-specific response to LPS exposure: more than 90% of identified mitochondrial membrane proteins were significant downregulated, whereas the majority of proteins from other organelles such as ER, Golgi and ribosome were upregulated. Moreover, we found that the expression of most receptors potentially involved in LPS signal pathway (CD14, toll-like receptor 4, CD11/CD18 complex) were substantially decreased, while the expression of molecules involved in LPS neutralization were enhanced after LPS challenge. Together, these findings could be of significance in understanding the mechanism of LPS tolerance and provide values for designing new approaches for regulating monocytic responses in sepsis patients.
dc.description.sponsorshipThis work was supported by grants from the Biomedical Research Council (BMRC: 07/1/22/19/531) and the Ministry of Education (ARC: T206B3211) of Singapore.
dc.publisherWiley-Blackwell
dc.titleStudy of monocyte membrane proteome perturbation during lipopolysaccharide-induced tolerance using iTRAQ-based quantitative proteomic approach
dc.typeArticle
dc.contributor.departmentBiosciences Core Lab
dc.contributor.departmentCore Labs
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentBioscience Program
dc.contributor.departmentIntegrative Systems Biology Lab
dc.identifier.journalPROTEOMICS
dc.contributor.institutionNanyang Technol Univ, Sch Biol Sci, Singapore, Singapore
dc.contributor.institutionHong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China
dc.contributor.institutionAgcy Sci Technol & Res, Singapore Immunol Network, Biopolis, Singapore
kaust.personZhang, Huoming
kaust.personWang, Yong
kaust.personRavasi, Timothy


This item appears in the following Collection(s)

Show simple item record