Microsatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci
AuthorsHarrison, Hugo B.
Saenz Agudelo, Pablo
Pratchett, Morgan S.
Berumen, Michael L.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Marine Science Program
Red Sea Research Center (RSRC)
Online Publication Date2015-03-20
Print Publication Date2015-09
Permanent link to this recordhttp://hdl.handle.net/10754/567096
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AbstractPopulation outbreaks of crown-of-thorns starfish (Acanthaster spp.) represent one of the most significant biological disturbances on Indo-Pacific coral reefs. Here, we combine 15 published and 11 newly isolated polymorphic microsatellite markers from the coral-eating starfish, A. cf. planci and describe their integration into four multiplex PCRs. All markers were polymorphic with a mean of 11.7 ± 1.9 SE alleles per locus and an average observed heterozygosity of 0.619 ± 0.049 SE across 195 genotyped individuals from the Great Barrier Reef. This multiplex assay provides an effective means of investigating the population dynamics of crown-of-thorns starfish and the initiation and spread of population outbreaks.
CitationMicrosatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci 2015, 7 (3):627 Conservation Genetics Resources
JournalConservation Genetics Resources