Microsatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci
AuthorsHarrison, Hugo B.
Saenz Agudelo, Pablo
Pratchett, Morgan S.
Berumen, Michael L.
KAUST DepartmentRed Sea Research Center (RSRC)
Permanent link to this recordhttp://hdl.handle.net/10754/567096
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AbstractPopulation outbreaks of crown-of-thorns starfish (Acanthaster spp.) represent one of the most significant biological disturbances on Indo-Pacific coral reefs. Here, we combine 15 published and 11 newly isolated polymorphic microsatellite markers from the coral-eating starfish, A. cf. planci and describe their integration into four multiplex PCRs. All markers were polymorphic with a mean of 11.7 ± 1.9 SE alleles per locus and an average observed heterozygosity of 0.619 ± 0.049 SE across 195 genotyped individuals from the Great Barrier Reef. This multiplex assay provides an effective means of investigating the population dynamics of crown-of-thorns starfish and the initiation and spread of population outbreaks.
CitationMicrosatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci 2015, 7 (3):627 Conservation Genetics Resources
PublisherSpringer Science + Business Media
JournalConservation Genetics Resources