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    Membrane-anchored MucR mediates nitrate-dependent regulation of alginate production in Pseudomonas aeruginosa

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    Type
    Article
    Authors
    Wang, Yajie
    Hay, Iain D.
    Rehman, Zahid Ur cc
    Rehm, Bernd H A
    KAUST Department
    Water Desalination and Reuse Research Center (WDRC)
    Date
    2015-04-29
    Online Publication Date
    2015-04-29
    Print Publication Date
    2015-09
    Permanent link to this record
    http://hdl.handle.net/10754/566131
    
    Metadata
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    Abstract
    Alginates exhibit unique material properties suitable for medical and industrial applications. However, if produced by Pseudomonas aeruginosa, it is an important virulence factor in infection of cystic fibrosis patients. The alginate biosynthesis machinery is activated by c-di-GMP imparted by the inner membrane protein, MucR. Here, it was shown that MucR impairs alginate production in response to nitrate in P. aeruginosa. Subsequent site-specific mutagenesis of MucR revealed that the second MHYT sensor motif (MHYT II, amino acids 121–124) of MucR sensor domain was involved in nitrate sensing. We also showed that both c-di-GMP synthesizing and degrading active sites of MucR were important for alginate production. Although nitrate and deletion of MucR impaired alginate promoter activity and global c-di-GMP levels, alginate yields were not directly correlated with alginate promoter activity or c-di-GMP levels, suggesting that nitrate and MucR modulate alginate production at a post-translational level through a localized pool of c-di-GMP. Nitrate increased pel promoter activity in the mucR mutant while in the same mutant the psl promoter activity was independent of nitrate. Nitrate and deletion of mucR did not impact on swarming motility but impaired attachment to solid surfaces. Nitrate and deletion of mucR promoted the formation of biofilms with increased thickness, cell density, and survival. Overall, this study provided insight into the functional role of MucR with respect to nitrate-mediated regulation of alginate biosynthesis. © 2015 Springer-Verlag Berlin Heidelberg
    Citation
    Wang, Y., Hay, I. D., Rehman, Z. U., & Rehm, B. H. A. (2015). Membrane-anchored MucR mediates nitrate-dependent regulation of alginate production in Pseudomonas aeruginosa. Applied Microbiology and Biotechnology, 99(17), 7253–7265. doi:10.1007/s00253-015-6591-4
    Publisher
    Springer Nature
    Journal
    Applied Microbiology and Biotechnology
    DOI
    10.1007/s00253-015-6591-4
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00253-015-6591-4
    Scopus Count
    Collections
    Articles; Water Desalination and Reuse Research Center (WDRC)

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