Isolation and characterization of polymorphic microsatellite loci for Siphamia tubifer Weber (Perciformes: Apogonidae)
KAUST DepartmentRed Sea Research Center (RSRC)
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AbstractThe cardinalfish Siphamia tubifer has been selected as a model for the study of genetic connectivity in reef-associated fishes among marine-protected-areas in Socotra Island in the northwestern Indian Ocean (part of the Socotra Archipelago, a UNESCO World Heritage Site since 2008). Twenty-six novel microsatellite markers are described for S. tubifer and are now available for studies on its genetic population structure. In a population sample from Socotra Island, the newly developed markers possessed between three and 20 alleles. Expected and observed heterozygosity ranged from 0.56–0.96 to 0.55–0.95, respectively. The markers did not show deviations from Hardy–Weinberg equilibrium and were not in linkage disequilibrium.
SponsorsThis study was funded by the research funding program LOEWE-Landes-Offensive zur Entwicklung Wissenschaftlich-okonomischer Exzellenz of Hesse's Ministry of Higher Education, Research, and the Arts and by the scientific research cooperation between King Abdulaziz University (KAU), Faculty of Marine Sciences (FMS), Jeddah, Saudi Arabia, and the Senckenberg Research Institute (SRI), Frankfurt, Germany, in the framework of the Red Sea Biodiversity Project (KAU Grant No. "D/1/432-DSR"). Friedhelm Krupp (Senckenberg Research Institute and Natural History Museum Frankfurt (SF) and Qatar Museum Authority) and Uwe Zajonz (Biodiversity and Climate Research Centre (BiK-F) and SF) are acknowledged for promoting this study as part of the BiK-F research program on tropical marine ecosystems. Mohamed Ahmer and Fouad Naseeb (Environmental Protection Authority, EPA, Socotra Branch) are thanked for supporting this research in the frame of the Memorandum of Agreement on Scientific and Technical Cooperation concerning the Socotra Archipelago between EPA and BiK-F. Thanks are also due to Fouad Naseeb and Maren Ziegler (BiK-F, SF) for help with field work and to Florian Wicker (SF) for assistance with lab work. Gavin Gouws (SAIAB) is acknowledged for comments on and improvements of the manuscript.
JournalConservation Genetics Resources