Embargo End Date2016-01-19
Permanent link to this recordhttp://hdl.handle.net/10754/566090
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AbstractElastic tissue was first described well over a hundred years ago and has since been identified in nearly every part of the body. In this review, we examine elastic tissue in the corneal stroma with some mention of other ocular structures which have been more thoroughly described in the past. True elastic fibers consist of an elastin core surrounded by fibrillin microfibrils. However, the presence of elastin fibers is not a requirement and some elastic tissue is comprised of non-elastin-containing bundles of microfibrils. Fibers containing a higher relative amount of elastin are associated with greater elasticity and those without elastin, with structural support. Recently it has been shown that the microfibrils, not only serve mechanical roles, but are also involved in cell signaling through force transduction and the release of TGF-β. A well characterized example of elastin-free microfibril bundles (EFMBs) is found in the ciliary zonules which suspend the crystalline lens in the eye. Through contraction of the ciliary muscle they exert enough force to reshape the lens and thereby change its focal point. It is believed that the molecules comprising these fibers do not turn-over and yet retain their tensile strength for the life of the animal. The mechanical properties of the cornea (strength, elasticity, resiliency) would suggest that EFMBs are present there as well. However, many authors have reported that, although present during embryonic and early postnatal development, EFMBs are generally not present in adults. Serial-block-face imaging with a scanning electron microscope enabled 3D reconstruction of elements in murine corneas. Among these elements were found fibers that formed an extensive network throughout the cornea. In single sections these fibers appeared as electron dense patches. Transmission electron microscopy provided additional detail of these patches and showed them to be composed of fibrils (~10nm diameter). Immunogold evidence clearly identified these fibrils as fibrillin EFMBs and EFMBs were also observed with TEM (without immunogold) in adult mammals of several species. Evidence of the presence of EFMBs in adult corneas will hopefully pique an interest in further studies that will ultimately improve our understanding of the cornea's biomechanical properties and its capacity to repair.
CitationHanlon, S. D., Behzad, A. R., Sakai, L. Y., & Burns, A. R. (2015). Corneal stroma microfibrils. Experimental Eye Research, 132, 198–207. doi:10.1016/j.exer.2015.01.014
SponsorsWe thank Evelyn Brown for her excellent help in preparation of EM specimens, and Paul Harris, Paul Landry, Siri Magadi, Nancy Shenoi, and Ian Smith for segmentation and 3D image reconstruction. Research results reported from our laboratory were obtained from projects supported by NEI grants: EY17120, EY007551 and HL116524.
JournalExperimental Eye Research
PubMed Central IDPMC4379971
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- Authors: White TL, Lewis PN, Young RD, Kitazawa K, Inatomi T, Kinoshita S, Meek KM
- Issue date: 2017 Jun
- Stretching stimulates fibulin-5 expression and controls microfibril bundles in human periodontal ligament cells.
- Authors: Nakashima K, Tsuruga E, Hisanaga Y, Ishikawa H, Sawa Y
- Issue date: 2009 Oct
- Immunohistochemical localization of elastin, fibrillins and microfibril-associated glycoprotein-1 in the developing periodontal ligament of the rat molar.
- Authors: Sugawara Y, Sawada T, Inoue S, Shibayama K, Yanagisawa T
- Issue date: 2010 Feb
- Microfibrils, elastin fibres and collagen fibres in the human intervertebral disc and bovine tail disc.
- Authors: Yu J, Tirlapur U, Fairbank J, Handford P, Roberts S, Winlove CP, Cui Z, Urban J
- Issue date: 2007 Apr
- [Expression of the elastic fibers components during the fœtal liver development].
- Authors: Villeneuve J, Pelluard-Nehmé F, Combe C, Carles D, Balabaud C, Bioulac-Sage P, Ripoche J, Lepreux S
- Issue date: 2010 Nov