Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae
dc.contributor.author | Aouida, Mustapha | |
dc.contributor.author | Li, Lixin | |
dc.contributor.author | Mahjoub, Ali | |
dc.contributor.author | Alshareef, Sahar | |
dc.contributor.author | Ali, Zahir | |
dc.contributor.author | Piatek, Agnieszka Anna | |
dc.contributor.author | Mahfouz, Magdy M. | |
dc.date.accessioned | 2015-08-12T09:00:57Z | |
dc.date.available | 2015-08-12T09:00:57Z | |
dc.date.issued | 2015-04-20 | |
dc.identifier.citation | Aouida, M., Li, L., Mahjoub, A., Alshareef, S., Ali, Z., Piatek, A., & Mahfouz, M. M. (2015). Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering, 120(4), 364–371. doi:10.1016/j.jbiosc.2015.02.017 | |
dc.identifier.issn | 13891723 | |
dc.identifier.pmid | 25907574 | |
dc.identifier.doi | 10.1016/j.jbiosc.2015.02.017 | |
dc.identifier.uri | http://hdl.handle.net/10754/566047 | |
dc.description.abstract | Targeted engineering of microbial genomes holds much promise for diverse biotechnological applications. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/Cas9 systems are capable of efficiently editing microbial genomes, including that of Saccharomyces cerevisiae. Here, we demonstrate the use of TALENs to edit the genome of S.cerevisiae with the aim of inducing the overproduction of fatty acids. Heterodimeric TALENs were designed to simultaneously edit the FAA1 and FAA4 genes encoding acyl-CoA synthetases in S.cerevisiae. Functional yeast double knockouts generated using these TALENs over-produce large amounts of free fatty acids into the cell. This study demonstrates the use of TALENs for targeted engineering of yeast and demonstrates that this technology can be used to stimulate the enhanced production of free fatty acids, which are potential substrates for biofuel production. This proof-of-principle study extends the utility of TALENs as excellent genome editing tools and highlights their potential use for metabolic engineering of yeast and other organisms, such as microalgae and plants, for biofuel production. © 2015 The Society for Biotechnology, Japan. | |
dc.publisher | Elsevier BV | |
dc.subject | Biofuel production | |
dc.subject | Free fatty acids | |
dc.subject | Genome engineering | |
dc.subject | Metabolic engineering | |
dc.subject | Synthetic biology | |
dc.subject | Transcription activator-like effector nucleases | |
dc.title | Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae | |
dc.type | Article | |
dc.contributor.department | Biological and Environmental Science and Engineering (BESE) Division | |
dc.contributor.department | Bioscience Program | |
dc.contributor.department | Center for Desert Agriculture | |
dc.contributor.department | General Materials | |
dc.contributor.department | Lab Consumables & Gases | |
dc.contributor.department | Laboratory for Genome Engineering | |
dc.contributor.department | Plant Science | |
dc.contributor.department | Plant Science Program | |
dc.contributor.department | Purchasing | |
dc.identifier.journal | Journal of Bioscience and Bioengineering | |
kaust.person | Aouida, Mustapha | |
kaust.person | Li, Lixin | |
kaust.person | Mahjoub, Ali | |
kaust.person | Ali, Zahir | |
kaust.person | Mahfouz, Magdy M. | |
kaust.person | Alshareef, Sahar | |
kaust.person | Piatek, Agnieszka Anna | |
dc.date.published-online | 2015-04-20 | |
dc.date.published-print | 2015-10 |
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Biological and Environmental Science and Engineering (BESE) Division
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Bioscience Program
For more information visit: https://bese.kaust.edu.sa/study/Pages/Bioscience.aspx -
Plant Science Program
For more information visit: https://bese.kaust.edu.sa/study/Pages/PS.aspx -
Center for Desert Agriculture