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dc.contributor.authorAouida, Mustapha
dc.contributor.authorLi, Lixin
dc.contributor.authorMahjoub, Ali
dc.contributor.authorAlshareef, Sahar
dc.contributor.authorAli, Zahir
dc.contributor.authorPiatek, Agnieszka Anna
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2015-08-12T09:00:57Z
dc.date.available2015-08-12T09:00:57Z
dc.date.issued2015-04-20
dc.identifier.citationAouida, M., Li, L., Mahjoub, A., Alshareef, S., Ali, Z., Piatek, A., & Mahfouz, M. M. (2015). Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering, 120(4), 364–371. doi:10.1016/j.jbiosc.2015.02.017
dc.identifier.issn13891723
dc.identifier.pmid25907574
dc.identifier.doi10.1016/j.jbiosc.2015.02.017
dc.identifier.urihttp://hdl.handle.net/10754/566047
dc.description.abstractTargeted engineering of microbial genomes holds much promise for diverse biotechnological applications. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/Cas9 systems are capable of efficiently editing microbial genomes, including that of Saccharomyces cerevisiae. Here, we demonstrate the use of TALENs to edit the genome of S.cerevisiae with the aim of inducing the overproduction of fatty acids. Heterodimeric TALENs were designed to simultaneously edit the FAA1 and FAA4 genes encoding acyl-CoA synthetases in S.cerevisiae. Functional yeast double knockouts generated using these TALENs over-produce large amounts of free fatty acids into the cell. This study demonstrates the use of TALENs for targeted engineering of yeast and demonstrates that this technology can be used to stimulate the enhanced production of free fatty acids, which are potential substrates for biofuel production. This proof-of-principle study extends the utility of TALENs as excellent genome editing tools and highlights their potential use for metabolic engineering of yeast and other organisms, such as microalgae and plants, for biofuel production. © 2015 The Society for Biotechnology, Japan.
dc.publisherElsevier BV
dc.subjectBiofuel production
dc.subjectFree fatty acids
dc.subjectGenome engineering
dc.subjectMetabolic engineering
dc.subjectSynthetic biology
dc.subjectTranscription activator-like effector nucleases
dc.titleTranscription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentGeneral Materials
dc.contributor.departmentLab Consumables & Gases
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Science Program
dc.contributor.departmentPurchasing
dc.identifier.journalJournal of Bioscience and Bioengineering
kaust.personAouida, Mustapha
kaust.personLi, Lixin
kaust.personMahjoub, Ali
kaust.personAli, Zahir
kaust.personMahfouz, Magdy M.
kaust.personAlshareef, Sahar
kaust.personPiatek, Agnieszka Anna
dc.date.published-online2015-04-20
dc.date.published-print2015-10


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