Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae
Type
ArticleAuthors
Aouida, Mustapha
Li, Lixin
Mahjoub, Ali

Alshareef, Sahar

Ali, Zahir

Piatek, Agnieszka Anna

Mahfouz, Magdy M.

KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
Center for Desert Agriculture
General Materials
Lab Consumables & Gases
Laboratory for Genome Engineering
Plant Science
Plant Science Program
Purchasing
Date
2015-04-20Online Publication Date
2015-04-20Print Publication Date
2015-10Permanent link to this record
http://hdl.handle.net/10754/566047
Metadata
Show full item recordAbstract
Targeted engineering of microbial genomes holds much promise for diverse biotechnological applications. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/Cas9 systems are capable of efficiently editing microbial genomes, including that of Saccharomyces cerevisiae. Here, we demonstrate the use of TALENs to edit the genome of S.cerevisiae with the aim of inducing the overproduction of fatty acids. Heterodimeric TALENs were designed to simultaneously edit the FAA1 and FAA4 genes encoding acyl-CoA synthetases in S.cerevisiae. Functional yeast double knockouts generated using these TALENs over-produce large amounts of free fatty acids into the cell. This study demonstrates the use of TALENs for targeted engineering of yeast and demonstrates that this technology can be used to stimulate the enhanced production of free fatty acids, which are potential substrates for biofuel production. This proof-of-principle study extends the utility of TALENs as excellent genome editing tools and highlights their potential use for metabolic engineering of yeast and other organisms, such as microalgae and plants, for biofuel production. © 2015 The Society for Biotechnology, Japan.Citation
Aouida, M., Li, L., Mahjoub, A., Alshareef, S., Ali, Z., Piatek, A., & Mahfouz, M. M. (2015). Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering, 120(4), 364–371. doi:10.1016/j.jbiosc.2015.02.017Publisher
Elsevier BVPubMed ID
25907574ae974a485f413a2113503eed53cd6c53
10.1016/j.jbiosc.2015.02.017
Scopus Count
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