• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Type
    Article
    Authors
    Aouida, Mustapha cc
    Li, Lixin
    Mahjoub, Ali cc
    Alshareef, Sahar cc
    Ali, Zahir cc
    Piatek, Agnieszka Anna cc
    Mahfouz, Magdy M. cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Program
    Center for Desert Agriculture
    General Materials
    Lab Consumables & Gases
    Laboratory for Genome Engineering
    Plant Science
    Plant Science Program
    Purchasing
    Date
    2015-04-20
    Online Publication Date
    2015-04-20
    Print Publication Date
    2015-10
    Permanent link to this record
    http://hdl.handle.net/10754/566047
    
    Metadata
    Show full item record
    Abstract
    Targeted engineering of microbial genomes holds much promise for diverse biotechnological applications. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/Cas9 systems are capable of efficiently editing microbial genomes, including that of Saccharomyces cerevisiae. Here, we demonstrate the use of TALENs to edit the genome of S.cerevisiae with the aim of inducing the overproduction of fatty acids. Heterodimeric TALENs were designed to simultaneously edit the FAA1 and FAA4 genes encoding acyl-CoA synthetases in S.cerevisiae. Functional yeast double knockouts generated using these TALENs over-produce large amounts of free fatty acids into the cell. This study demonstrates the use of TALENs for targeted engineering of yeast and demonstrates that this technology can be used to stimulate the enhanced production of free fatty acids, which are potential substrates for biofuel production. This proof-of-principle study extends the utility of TALENs as excellent genome editing tools and highlights their potential use for metabolic engineering of yeast and other organisms, such as microalgae and plants, for biofuel production. © 2015 The Society for Biotechnology, Japan.
    Citation
    Aouida, M., Li, L., Mahjoub, A., Alshareef, S., Ali, Z., Piatek, A., & Mahfouz, M. M. (2015). Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering, 120(4), 364–371. doi:10.1016/j.jbiosc.2015.02.017
    Publisher
    Elsevier BV
    Journal
    Journal of Bioscience and Bioengineering
    DOI
    10.1016/j.jbiosc.2015.02.017
    PubMed ID
    25907574
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.jbiosc.2015.02.017
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Center for Desert Agriculture

    entitlement

    Related articles

    • Enhancement of free fatty acid production in Saccharomyces cerevisiae by control of fatty acyl-CoA metabolism.
    • Authors: Chen L, Zhang J, Lee J, Chen WN
    • Issue date: 2014 Aug
    • The Acyl-CoA synthetases encoded within FAA1 and FAA4 in Saccharomyces cerevisiae function as components of the fatty acid transport system linking import, activation, and intracellular Utilization.
    • Authors: Faergeman NJ, Black PN, Zhao XD, Knudsen J, DiRusso CC
    • Issue date: 2001 Oct 5
    • Biosynthesis of Long-Chain ω-Hydroxy Fatty Acids by Engineered Saccharomyces cerevisiae.
    • Authors: Liu J, Zhang C, Lu W
    • Issue date: 2019 Apr 24
    • Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation Using Csy4 in Saccharomyces cerevisiae.
    • Authors: Ferreira R, Skrekas C, Nielsen J, David F
    • Issue date: 2018 Jan 19
    • Redirection of lipid flux toward phospholipids in yeast increases fatty acid turnover and secretion.
    • Authors: Ferreira R, Teixeira PG, Siewers V, Nielsen J
    • Issue date: 2018 Feb 6
    DSpace software copyright © 2002-2023  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.