Microbial Community Composition and Ultrastructure of Granules from a Full-Scale Anammox Reactor
KAUST DepartmentAdvanced Nanofabrication, Imaging and Characterization Core Lab
Biological and Environmental Sciences and Engineering (BESE) Division
Environmental Science and Engineering Program
Imaging and Characterization Core Lab
Water Desalination and Reuse Research Center (WDRC)
Online Publication Date2014-12-11
Print Publication Date2015-07
Permanent link to this recordhttp://hdl.handle.net/10754/565996
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AbstractGranules in anammox reactors contain besides anammox bacteria other microbial communities whose identity and relationship with the anammox bacteria are not well understood. High calcium concentrations are often supplied to anammox reactors to obtain sufficient bacterial aggregation and biomass retention. The aim of this study was to provide the first characterization of bacterial and archaeal communities in anammox granules from a full-scale anammox reactor and to explore on the possible role of calcium in such aggregates. High magnification imaging using backscattered electrons revealed that anammox bacteria may be embedded in calcium phosphate precipitates. Pyrosequencing of 16S rRNA gene fragments showed, besides anammox bacteria (Brocadiacea, 32 %), substantial numbers of heterotrophic bacteria Ignavibacteriacea (18 %) and Anaerolinea (7 %) along with heterotrophic denitrifiers Rhodocyclacea (9 %), Comamonadacea (3 %), and Shewanellacea (3 %) in the granules. It is hypothesized that these bacteria may form a network in which heterotrophic denitrifiers cooperate to achieve a well-functioning denitrification system as they can utilize the nitrate intrinsically produced by the anammox reaction. This network may provide a niche for the proliferation of archaea. Hydrogenotrophic methananogens, which scavenge the key fermentation product H2, were the most abundant archaea detected. Cells resembling the polygon-shaped denitrifying methanotroph Candidatus Methylomirabilis oxyfera were observed by electron microscopy. It is hypothesized that the anammox process in a full-scale reactor triggers various reactions overall leading to efficient denitrification and a sink of carbon as biomass in anammox granules.
CitationGonzalez-Gil, G., Sougrat, R., Behzad, A. R., Lens, P. N. L., & Saikaly, P. E. (2014). Microbial Community Composition and Ultrastructure of Granules from a Full-Scale Anammox Reactor. Microbial Ecology, 70(1), 118–131. doi:10.1007/s00248-014-0546-7
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