Show simple item record

dc.contributor.authorHouben, Diane
dc.contributor.authorDemangel, Caroline
dc.contributor.authorVan Ingen, Jakko
dc.contributor.authorPerez, Jorge
dc.contributor.authorBaldeón, Lucy R.
dc.contributor.authorAbdallah, Abdallah
dc.contributor.authorCaleechurn, Laxmee
dc.contributor.authorBottai, Daria
dc.contributor.authorVan Zon, Maaike
dc.contributor.authorDe Punder, Karin
dc.contributor.authorVan Der Laan, Tridia
dc.contributor.authorKant, Arie
dc.contributor.authorBossers-De Vries, Ruth
dc.contributor.authorWillemsen, Peter Th J
dc.contributor.authorBitter, Wilbert M.
dc.contributor.authorVan Soolingen, Dick
dc.contributor.authorBrosch, Roland
dc.contributor.authorVan Der Wel, Nicole N.
dc.contributor.authorPeters, Peter J.
dc.date.accessioned2015-08-12T08:57:39Z
dc.date.available2015-08-12T08:57:39Z
dc.date.issued2012-05-08
dc.identifier.issn14625814
dc.identifier.pmid22524898
dc.identifier.doi10.1111/j.1462-5822.2012.01799.x
dc.identifier.urihttp://hdl.handle.net/10754/565974
dc.description.abstractMycobacterium species, including Mycobacterium tuberculosis and Mycobacterium leprae, are among the most potent human bacterial pathogens. The discovery of cytosolic mycobacteria challenged the paradigm that these pathogens exclusively localize within the phagosome of host cells. As yet the biological relevance of mycobacterial translocation to the cytosol remained unclear. In this current study we used electron microscopy techniques to establish a clear link between translocation and mycobacterial virulence. Pathogenic, patient-derived mycobacteria species were found to translocate to the cytosol, while non-pathogenic species did not. We were further able to link cytosolic translocation with pathogenicity by introducing the ESX-1 (type VII) secretion system into the non-virulent, exclusively phagolysosomal Mycobacterium bovis BCG. Furthermore, we show that translocation is dependent on the C-terminus of the early-secreted antigen ESAT-6. The C-terminal truncation of ESAT-6 was shown to result in attenuation in mice, again linking translocation to virulence. Together, these data demonstrate the molecular mechanism facilitating translocation of mycobacteria. The ability to translocate from the phagolysosome to the cytosol is with this study proven to be biologically significant as it determines mycobacterial virulence. © 2012 Blackwell Publishing Ltd.
dc.publisherWiley
dc.titleESX-1-mediated translocation to the cytosol controls virulence of mycobacteria
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalCellular Microbiology
kaust.personAbdallah, Abdallah
kaust.personBossers-De Vries, Ruth
kaust.personWillemsen, Peter Th J


This item appears in the following Collection(s)

Show simple item record