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    The RNA-binding protein HOS5 and serine/arginine-rich proteins RS40 and RS41 participate in miRNA biogenesis in Arabidopsis

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    Type
    Article
    Authors
    Chen, Tao
    Cui, Peng cc
    Xiong, Liming cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Plant Science
    Date
    2015-07-30
    Online Publication Date
    2015-07-30
    Print Publication Date
    2015-09-30
    Permanent link to this record
    http://hdl.handle.net/10754/565054
    
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    Abstract
    MicroRNAs are a class of small regulatory RNAs that are generated from primary miRNA (pri-miRNA) transcripts with a stem-loop structure. Accuracy of the processing of pri-miRNA into mature miRNA in plants can be enhanced by SERRATE (SE) and HYPONASTIC LEAVES 1 (HYL1). HYL1 activity is regulated by the FIERY2 (FRY2)/RNA polymerase II C-terminal domain phosphatase-like 1 (CPL1). Here, we discover that HIGH OSMOTIC STRESS GENE EXPRESSION 5 (HOS5) and two serine/arginine-rich splicing factors RS40 and RS41, previously shown to be involved in pre-mRNA splicing, affect the biogenesis of a subset of miRNA. These proteins are required for correct miRNA strand selection and the maintenance of miRNA levels. FRY2 dephosphorylates HOS5 whose phosphorylation status affects its subnuclear localization. HOS5 and the RS proteins bind both intronless and intron-containing pri-miRNAs. Importantly, all of these splicing-related factors directly interact with both HYL1 and SE in nuclear splicing speckles. Our results indicate that these splicing factors are directly involved in the biogenesis of a group of miRNA.
    Citation
    The RNA-binding protein HOS5 and serine/arginine-rich proteins RS40 and RS41 participate in miRNA biogenesis in Arabidopsis 2015:gkv751 Nucleic Acids Research
    Publisher
    Oxford University Press (OUP)
    Journal
    Nucleic Acids Research
    DOI
    10.1093/nar/gkv751
    PubMed ID
    26227967
    Additional Links
    http://nar.oxfordjournals.org/lookup/doi/10.1093/nar/gkv751
    ae974a485f413a2113503eed53cd6c53
    10.1093/nar/gkv751
    Scopus Count
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    Articles; Biological and Environmental Science and Engineering (BESE) Division

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