Improved detection limits of bacterial endotoxins using new type of planar interdigital sensors
Type
Conference PaperAuthors
Syaifudin, A. R MohdZia, Asif I.
Mukhopadhyay, Subhas Chandra
Yu, Paklam
Gooneratne, Chinthaka Pasan
Kosel, Jürgen

KAUST Department
Sensing, Magnetism and Microsystems LabComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Electrical Engineering Program
Date
2012-10Permanent link to this record
http://hdl.handle.net/10754/564615
Metadata
Show full item recordAbstract
New types of planar interdigital sensors were fabricated by photolithography and etching techniques on a Silicon/Silicon Dioxide (Si/SiO2) wafer (single side polished). The sensors were then coated with APTES (3-aminopropyltrietoxysilane) a cross linker used to bind Polymyxin B (PmB) molecules on electrodes surface. PmB is an antimicrobial peptide produced by the Gram-positive bacterium-Bacillus which has specific binding properties to Lipopolysaccharide (LPS). This paper will discuss the fabrication process, coating and immobilization procedures and analysis of sensors' performance based on Impedance Spectroscopy method. The sensor sensitivity was compared to standard ToxinSensor Chromogenic LAL Endotoxin Assay Kit for verification. © 2012 IEEE.Journal
2012 IEEE SensorsConference/Event name
11th IEEE SENSORS 2012 ConferenceISBN
9781457717659ae974a485f413a2113503eed53cd6c53
10.1109/ICSENS.2012.6411193