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dc.contributor.authorZhang, Huoming
dc.contributor.authorWong, Yuehim
dc.contributor.authorWang, Hao
dc.contributor.authorChen, Zhangfan
dc.contributor.authorArellano, Shawn M.
dc.contributor.authorRavasi, Timothy
dc.contributor.authorQian, Pei-Yuan
dc.date.accessioned2015-08-04T06:24:52Z
dc.date.available2015-08-04T06:24:52Z
dc.date.issued2011-01-07
dc.identifier.citationZhang, H., Wong, Y. H., Wang, H., Chen, Z., Arellano, S. M., Ravasi, T., & Qian, P.-Y. (2011). Quantitative Proteomics Identify Molecular Targets That Are Crucial in Larval Settlement and Metamorphosis ofBugula neritina. Journal of Proteome Research, 10(1), 349–360. doi:10.1021/pr100817v
dc.identifier.issn15353893
dc.identifier.pmid21090758
dc.identifier.doi10.1021/pr100817v
dc.identifier.urihttp://hdl.handle.net/10754/564359
dc.description.abstractThe marine invertebrate Bugula neritina has a biphasic life cycle that consists of a swimming larval stage and a sessile juvenile and adult stage. The attachment of larvae to the substratum and their subsequent metamorphosis have crucial ecological consequences. Despite many studies on this species, little is known about the molecular mechanism of these processes. Here, we report a comparative study of swimming larvae and metamorphosing individuals at 4 and 24 h postattachment using label-free quantitative proteomics. We identified more than 1100 proteins at each stage, 61 of which were differentially expressed. Specifically, proteins involved in energy metabolism and structural molecules were generally down-regulated, whereas proteins involved in transcription and translation, the extracellular matrix, and calcification were strongly up-regulated during metamorphosis. Many tightly regulated novel proteins were also identified. Subsequent analysis of the temporal and spatial expressions of some of the proteins and an assay of their functions indicated that they may have key roles in metamorphosis of B. neritina. These findings not only provide molecular evidence with which to elucidate the substantial changes in morphology and physiology that occur during larval attachment and metamorphosis but also identify potential targets for antifouling treatment. © 2011 American Chemical Society.
dc.description.sponsorshipWe thank Jin Sun for his invaluable discussion and Cherry Hoi Ting Kwan for her comments on the manuscript. This study was supported by award SA-C0040/UK-C0016 from the King Abdullah University of Science and Technology, and grants (N-HKUST602/09 662408 and AoE/P-04/04-II) from the Research Grants Council of the Hong Kong Special Administrative Region to P.Y.Q.
dc.publisherAmerican Chemical Society (ACS)
dc.subjectBiofouling
dc.subjectBugula neritina
dc.subjectLabel-free quantitative proteomics
dc.subjectLarval attachment and metamorphosis
dc.titleQuantitative proteomics identify molecular targets that are crucial in larval settlement and metamorphosis of bugula neritina
dc.typeArticle
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentBioscience Core Lab
dc.contributor.departmentMaterials Science and Engineering Program
dc.contributor.departmentIntegrative Systems Biology Lab
dc.identifier.journalJournal of Proteome Research
dc.contributor.institutionHong Kong University of Science and Technology, Hong Kong
kaust.personZhang, Huoming
kaust.personWang, Hao
kaust.personRavasi, Timothy
kaust.personWong, Yuehim
kaust.personChen, Zhangfan
kaust.personArellano, Shawn M.
kaust.personQian, Pei-Yuan


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